Evidence map›Paper›PMID 40437035›Full record

ArticleScientific reports2025

Brown juice processed from alfalfa green biomass as a source of phytohormones and saponins.

Nóra Bákonyi, Döme Barna, Wildan Suhartini, Zoltán Cziáky, Makleit Péter, Tarek Alshaal, Miklós Gábor Fári, Éva Domokos-Szabolcsy

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nóra BákonyiInstitute of Applied Plant Biology, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Böszörményi Street 138, Debrecen, 4032, Hungary. nbakonyi@agr.unideb.hu.
Döme BarnaInstitute of Applied Plant Biology, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Böszörményi Street 138, Debrecen, 4032, Hungary.
Wildan SuhartiniInstitute of Applied Plant Biology, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Böszörményi Street 138, Debrecen, 4032, Hungary.
Zoltán CziákyAgricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyíregyháza, 4407, Hungary.
Makleit PéterInstitute of Applied Plant Biology, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Böszörményi Street 138, Debrecen, 4032, Hungary.
Tarek AlshaalInstitute of Applied Plant Biology, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Böszörményi Street 138, Debrecen, 4032, Hungary.
Miklós Gábor FáriInstitute of Applied Plant Biology, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Böszörményi Street 138, Debrecen, 4032, Hungary.
Éva Domokos-SzabolcsyInstitute of Applied Plant Biology, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Böszörményi Street 138, Debrecen, 4032, Hungary.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The valorization of by-products from green biorefineries is critical for environmental sustainability and economic viability. During the extraction of leaf proteins from alfalfa green biomass, significant volumes of brown juice (BJ) are generated. This study assessed the phytohormonal profile and anti-nutritional elements in BJ post-lacto-fermentation, examining its potential as a plant biostimulant. Fresh BJ was lacto-fermented using Pediococcus acidilactici, Lactobacillus paracasei, and Lactobacillus plantarum at 35 °C for 48 h. Phytochemical analysis via HPLC-MS/MS identified 18 saponins in BJ, with only medicagenic acid remaining post-fermentation, whose concentration increased 39-fold. Additionally, 23 plant hormone derivatives were quantified, with significant increases observed in indole-3-acetic acid, gibberellin GA1, jasmonic acid, and abscisic acid, while others like p-coumaric acid, caffeic acid, and ferulic acid decreased. These results demonstrate lacto-fermentation's ability to stabilize BJ, reduce haemolytic saponins, and enhance phytohormone concentrations, supporting its utility as a sustainable plant growth enhancer.

Indexed as

Medicago sativaPlant Growth RegulatorsSaponinsBiomassChromatography, High Pressure LiquidFermentationPlant LeavesTandem Mass SpectrometryPlant Growth RegulatorsSaponinsAlfalfa brown juiceLacto-fermentationMedicagenic acidPlant hormonesSaponins

Identifiers

PMID40437035
PMCPMC12120019

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.