Evidence map›Paper›PMID 40436869›Full record

ArticleNature communications2025

Divergent molecular pathways govern temperature-dependent wheat stem rust resistance genes.

Tim C Hewitt, Keshav Sharma, Jianping Zhang, Chunhong Chen, Prabin Bajgain, Dhara Bhatt, Smriti Singh, Pablo D Olivera Firpo, Jun Yang, Qiaoli Wang and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Identification and molecular mapping of a new Hessian fly resistance gene in the spring barley variety 'Golf'.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Tim C Hewitt *CSIRO Agriculture & Food, Canberra, ACT, Australia.ORCID http://orcid.org/0000-0002-4888-7216
Keshav Sharma *USDA-ARS, Cereal Disease Laboratory, St. Paul, MN, USA.
Jianping Zhang *University of Sydney, Plant Breeding Institute, School of Life and Environmental Sciences, Cobbitty, NSW, Australia.ORCID http://orcid.org/0000-0003-2488-5723
Chunhong ChenCSIRO Agriculture & Food, Canberra, ACT, Australia.ORCID http://orcid.org/0000-0001-7813-2415
Prabin BajgainDepartment of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN, USA.
Dhara BhattCSIRO Agriculture & Food, Canberra, ACT, Australia.
Smriti SinghUniversity of Sydney, Plant Breeding Institute, School of Life and Environmental Sciences, Cobbitty, NSW, Australia.
Pablo D Olivera FirpoUSDA-ARS, Cereal Disease Laboratory, St. Paul, MN, USA.
Jun YangCSIRO Agriculture & Food, Canberra, ACT, Australia.
Qiaoli WangCentre for Crop Genome Engineering and College of Agronomy, Longzi Lake Campus, Henan Agricultural University, Zhengzhou, China.
Narayana UpadhyayaCSIRO Agriculture & Food, Canberra, ACT, Australia.ORCID http://orcid.org/0000-0002-3052-0416
Curtis PozniakCrop Development Centre and Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada.ORCID http://orcid.org/0000-0002-7536-3856
Robert McIntoshUniversity of Sydney, Plant Breeding Institute, School of Life and Environmental Sciences, Cobbitty, NSW, Australia. robert.mcintosh@sydney.edu.au.
Evans LagudahCSIRO Agriculture & Food, Canberra, ACT, Australia. evans.lagudah@csiro.au.ORCID http://orcid.org/0000-0002-6234-1789
Peng ZhangUniversity of Sydney, Plant Breeding Institute, School of Life and Environmental Sciences, Cobbitty, NSW, Australia. peng.zhang@sydney.edu.au.ORCID http://orcid.org/0000-0002-4191-1068
Matthew N RouseUSDA-ARS, Cereal Disease Laboratory, St. Paul, MN, USA. matthew.rouse@usda.gov.ORCID http://orcid.org/0000-0001-7763-8203

Funding

Department of Education and Training | Australian Research Council (ARC) IE230100282
6 · The paper itself

Abstract

The wheat stem rust pathogen Puccinia graminis f. sp. tritici (Pgt) causes severe crop losses worldwide. Several stem rust resistance (Sr) genes exhibit temperature-dependent immune responses. Sr6-mediated resistance is enhanced at lower temperatures, whereas Sr13 and Sr21 resistances are enhanced at higher temperatures. Here, we clone Sr6 using mutagenesis and resistance gene enrichment and sequencing (MutRenSeq), identifying it to encode a nucleotide-binding leucine-rich repeat (NLR) protein with an integrated BED domain. Sr6 temperature sensitivity is also transferred to wheat plants transformed with the Sr6 gene. Differential gene expression analysis of near-isogenic lines inoculated with Pgt at varying temperatures reveals that genes upregulated in the low-temperature-effective Sr6 response differ from those upregulated in the high-temperature-effective responses associated with Sr13 and Sr21. These findings highlight divergent molecular pathways involved in temperature-sensitive immunity and inform future strategies for deployment and engineering of genetic resistance in response to a changing climate.

Indexed as

Disease ResistancePlant DiseasesPucciniaTriticumBasidiomycotaGene Expression Regulation, PlantGenes, PlantNLR ProteinsPlant ProteinsPlant StemsTemperatureNLR ProteinsPlant Proteins

Identifiers

PMID40436869
PMCPMC12119863

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.