ArticleNature communications2025
An optimized disease resistance gene cloning workflow for wheat.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Discovering putative novel stem rust resistance loci in wheat genetic resources.The plant genome · 2026Article
- The emerging role of kinase fusion proteins in cereal immunity.Nature genetics · 2026Review
- Development and application of duplex and triplex assays for simultaneous detection of resistance genes to leaf rust, Fusarium head blight, powdery mildew, Septoria tritici blotch, eyspot, stem rust and yellow rust in wheat.Journal of applied genetics · 2026Article
- Progress in Molecular Plant Science (2023-2025).Current issues in molecular biology · 2025Article
- Wheat Plasma Membrane Receptors: Orchestrating Immunity and Bridging to Crop Improvement.Current issues in molecular biology · 2025Review
- Genome-wide dissection of lesion mimic mutations in wheat: identification and functional characterization of candidate genes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
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Authors and funding
10 authors.
Funding
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Abstract
The cloning of disease resistance genes in wheat has been disproportionately slow, tedious and costly because of the large and complex genome. Wheat gene cloning projects in the late 1990s and early 2000s were multi-year endeavors, often spanning a decade or longer. The development of genomics-assisted gene cloning tools and speed breeding have significantly accelerated gene cloning in wheat over the past years. Here, we present an optimized high-throughput disease resistance gene cloning workflow that allows to identify causal genes in less than six months. As a proof-of-principle, we clone the stem rust resistance gene Sr6, which has been a historically relevant source of resistance to confine a major stem rust outbreak in North America in the mid-20
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