Evidence map›Paper›PMID 40436327›Full record

ArticleToxicology in vitro : an international journal published in association with BIBRA2025

Development of a TK6-derived cell line expressing four human cytochrome P450s for genotoxicity testing.

Xilin Li, Yuhan Wang, Hannah Xu, Xiaobo He, Si Chen, Xiaoqing Guo, Mugimane G Manjanatha, Tong Zhou, Jessica Bonzo, Nan Mei

Abstract read
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In one paragraph

Article in Toxicology in vitro : an international journal published in association with BIBRA, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xilin LiNational Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.
Yuhan WangNational Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.
Hannah XuNational Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.
Xiaobo HeNational Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.
Si ChenNational Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.
Xiaoqing GuoNational Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.
Mugimane G ManjanathaNational Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.
Tong ZhouCenter for Veterinary Medicine, U.S. Food and Drug Administration, Rockville, MD 20855, USA.
Jessica BonzoCenter for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD 20993, USA.
Nan MeiNational Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.. Electronic address: nan.mei@fda.hhs.gov.

Funding

Intramural FDA HHS FD999999
6 · The paper itself

Abstract

Metabolism is essential for in vitro genotoxicity testing. We previously developed a panel of TK6 cell lines, each expressing one of 14 human cytochrome P450 (CYP) enzymes, demonstrating their ability to effectively bioactivate indirect genotoxicants without relying on a rodent liver S9 fraction. In the present study, we extended this work by developing a TK6 cell line co-expressing four human CYP enzymes, including CYP2A6, CYP2E1, CYP2C19, and CYP3A4 (designated as TK6-4CYP), and subsequently assessed its capability to metabolize and activate pro-genotoxicants. Human lymphoblastoid TK6 cells were sequentially transduced with lentiviral vectors carrying CYP2A6/2E1 and CYP2C19/3A4, resulting in more than a 2

Indexed as

Cytochrome P-450 Enzyme SystemMutagensCell LineHumansMutagenicity TestsCytochrome P-450 Enzyme SystemMutagensBiotransformationChromosomal damageCytochrome P450DNA damageTK6 cells

Identifiers

PMID40436327
PMCPMC12359084

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.