Evidence map›Paper›PMID 40435860›Full record

ArticleEnvironment international2025

Dose-response assessment of dipentyl phthalate effects on testosterone production and morphogenesis of late-gestation fetal rat testis.

Maansi V Gupta, Justin M Conley, Christy Lambright, Logan F Chin, Susan J Hall, L Earl Gray, Daniel J Spade

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Environment international, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Maansi V GuptaDepartment of Pathology and Laboratory Medicine, Brown University, Box G-E5, Providence, RI 02912, USA. Electronic address: maansi_gupta@alumni.brown.edu.
Justin M ConleyOffice of Research & Development/Center for Public Health and Environmental Assessment, U.S. Environmental Protection Agency, Mail Code B305-01, Research Triangle Park, NC 27711, USA. Electronic address: conley.justin@epa.gov.
Christy LambrightOffice of Research & Development/Center for Public Health and Environmental Assessment, U.S. Environmental Protection Agency, Mail Code B305-01, Research Triangle Park, NC 27711, USA. Electronic address: lambright.christy@epa.gov.
Logan F ChinDepartment of Pathology and Laboratory Medicine, Brown University, Box G-E5, Providence, RI 02912, USA. Electronic address: logan_chin@alumni.brown.edu.
Susan J HallDepartment of Pathology and Laboratory Medicine, Brown University, Box G-E5, Providence, RI 02912, USA. Electronic address: susan_hall@brown.edu.
L Earl GrayOffice of Research & Development/Center for Public Health and Environmental Assessment, U.S. Environmental Protection Agency, Mail Code B305-01, Research Triangle Park, NC 27711, USA. Electronic address: gray.earl@epa.gov.
Daniel J SpadeDepartment of Pathology and Laboratory Medicine, Brown University, Box G-E5, Providence, RI 02912, USA. Electronic address: daniel_spade@brown.edu.

Funding

CRR LIGAND ASSAY AND ANALYSIS CORER24HD102061 · NICHD · UNIVERSITY OF VIRGINIA · PI HAISENLEDER, DANIEL J. · 2019 to 2023
$2.2M
Retinoic Acid Signaling Disruption by Phthalates in Human and Rodent Fetal TestisR00ES025231 · NIEHS · BROWN UNIVERSITY · PI SPADE, DANIEL JAMES · 2018 to 2020
$725k
NICHD NIH HHS R24 HD102061NIEHS NIH HHS R00 ES025231
6 · The paper itself

Abstract

Dipentyl phthalate (DPeP) is a potent male reproductive toxicant that reduces fetal testicular testosterone production and induces abnormal fetal testis morphology, including multinucleated germ cells (MNGs). We aimed to test whether testosterone production, MNG density, or gene expression would be most sensitive to DPeP exposure and to determine which transcriptomic processes are initiated at the lowest DPeP dose. Timed pregnant Sprague Dawley rats were exposed to 0, 1, 11, 33, 100, or 300 mg DPeP/kg/d by oral gavage from GD 17-21. For comparison to DPeP, additional rats were exposed to vinclozolin, prochloraz, acetaminophen, mono-(2-ethylhexyl) tetrabromophthalate, and dexamethasone. Testosterone production was measured using an ex vivo culture assay, MNGs were quantified on testis sections, and fetal testes were used for RNA-seq, immunofluorescence, and in situ hybridization. Benchmark dose (BMD) analysis was used to compare apical endpoints and gene expression. DPeP dose-dependently reduced testosterone production and increased MNG density. ED

Indexed as

Phthalic AcidsTestisTestosteroneAnimalsDose-Response Relationship, DrugFemaleFetusMaleMorphogenesisPregnancyRatsRats, Sprague-DawleyPhthalic AcidsTestosteroneDevelopmentGerm cellsPhthalatesTestisTestosterone

Identifiers

PMID40435860
PMCPMC12163936

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.