Evidence map›Paper›PMID 40435813›Full record

ArticleBiomaterials2025

Graphene-polymer nanofibers enable optically induced electrical responses in stem cell-derived electrically excitable cells and brain organoids.

Erin LaMontagne, Alex Savchenko, Gisselle Gonzalez, Ritwik Vatsyayan, Blanca Martin-Burgos, Francesca Puppo, Diogo Biagi, Fabio Papes, Shadi A Dayeh, Alysson R Muotri and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Erin LaMontagneShu Chien-Gene Lay Department of Bioengineering, La Jolla, CA, 92093, USA.
Alex SavchenkoNanotools Bioscience, La Jolla, CA, 92093, USA.
Gisselle GonzalezShu Chien-Gene Lay Department of Bioengineering, La Jolla, CA, 92093, USA.
Ritwik VatsyayanDepartment of Electrical and Computer Engineering, La Jolla, CA, 92093, USA.
Blanca Martin-BurgosNeurosciences Graduate Program, La Jolla, CA, 92093, USA.
Francesca PuppoDepartment of Pediatrics, La Jolla, CA, 92093, USA.
Diogo BiagiDepartment of Pediatrics, La Jolla, CA, 92093, USA.
Fabio PapesDepartment of Pediatrics, La Jolla, CA, 92093, USA.
Shadi A DayehDepartment of Electrical and Computer Engineering, La Jolla, CA, 92093, USA.
Alysson R MuotriNeurosciences Graduate Program, La Jolla, CA, 92093, USA; Department of Pediatrics, La Jolla, CA, 92093, USA; Departmentof Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA, 92093, USA; Sanford Stem Cell Institute, La Jolla, CA, 92037, USA. Electronic address: muotri@ucsd.edu.
Adam J EnglerShu Chien-Gene Lay Department of Bioengineering, La Jolla, CA, 92093, USA; Sanford Stem Cell Institute, La Jolla, CA, 92037, USA. Electronic address: aengler@ucsd.edu.

Funding

Proteogenetics of Autism Spectrum DisordersR01MH100175 · NIMH · SCRIPPS RESEARCH INSTITUTE, THE · PI MUOTRI, ALYSSON R., YATES III, JOHN R · 2013 to 2025
$7.4M
Novel SETD5-based Molecular Mechanisms and Therapeutic Tools to Understand and Revert Neuronal Dysfunction Associated with Intellectual disability and AutismR01MH127077 · NIMH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Alon Goren, Alysson R. Muotri · 2022 to 2026
$3.9M
Establishment of a causal link between AD and L1 retrotransposonsR01AG078959 · NIA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Alysson R. Muotri · 2022 to 2026
$3.9M
Brain pathology and function in a chronic mouse model of ZIKV transmissionR01NS105969 · NINDS · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI MUOTRI, ALYSSON R., OLSON, STEVEN H · 2019 to 2023
$3.3M
Investigating neurodevelopmental toxicity of perfluoroalkyl acids and their derivatives in human brain organoids modelsR01ES033636 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LILIA M IAKOUCHEVA, Alysson R. Muotri · 2022 to 2026
$3.0M
Integrative functional genomic study of pathways impacted by recurrent autism CNVR01MH108528 · NIMH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI IAKOUCHEVA, LILIA M, MUOTRI, ALYSSON R. · 2016 to 2020
$2.6M
Biophysical Interrogation of Signals that Drive GBM InvasionR01NS116802 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ENGLER, ADAM J, FURNARI, FRANK · 2020 to 2024
$2.3M
The Impact of hiPSC-Derived Microglia in Human Brain Development in Health and DiseaseR01NS123642 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MUOTRI, ALYSSON R. · 2021 to 2025
$2.2M
Investigation of Pitt-Hopkins Syndrome pathophysiology using a human modelR01MH123828 · NIMH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MUOTRI, ALYSSON R. · 2021 to 2025
$1.9M
Cortical organoid models to study autism-associated 16p.11.2.CNVR56MH128365 · NIMH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI IAKOUCHEVA, LILIA M, MUOTRI, ALYSSON R. · 2022 to 2022
$706k
Illumina NovaSeq 6000 Sequencing SystemS10OD026929 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JEPSEN, KRISTEN LYNN · 2019 to 2019
$600k
Non-genetic optical stimulation platform for the next-generation neuroscience drug discovery assaysR43MH124563 · NIMH · NANOTOOLS BIOSCIENCE · PI SAVTCHENKO, ALEX · 2021 to 2021
$350k
FDA HHS U01 FD006676NIA NIH HHS R01 AG078959NIEHS NIH HHS R01 ES033636NIH HHS S10 OD026929NIMH NIH HHS R01 MH100175NIMH NIH HHS R01 MH108528NIMH NIH HHS R01 MH123828NIMH NIH HHS R01 MH127077NIMH NIH HHS R43 MH124563NIMH NIH HHS R56 MH128365NINDS NIH HHS R01 NS105969NINDS NIH HHS R01 NS116802NINDS NIH HHS R01 NS123642
6 · The paper itself

Abstract

Human pluripotent stem cell (hPSC)-derived electrically excitable cells provide a unique window into development, but they remain electrically immature partially due to the lack of chronic stimulation. Here, we fabricated electrospun polymer nanofibers containing light-reactive reduced graphene oxide (rGO) as part of a new classes of on-demand, electrically active biomaterials to enhance cell function. Fiber size, stiffness, and electrical conductivity varied with rGO concentration, which impacted hPSC-derived cardiomyocyte and neuron responses; with acute light stimulation, cardiomyocytes exhibited increased, synchronous calcium handling. Long-term, daily nanofiber light stimulation improves brain organoid electrical activity and activates photoreceptor pathways. This work outlines a tunable method where electrical cell functions can be titrated with rGO fibers and light stimulation, and it suggests that repetitive light stimulation may provide a novel method for retinal differentiation.

Indexed as

BrainGraphiteNanofibersOrganoidsPluripotent Stem CellsPolymersCalciumCell DifferentiationElectric ConductivityHumansLightMyocytes, CardiacNeuronsCalciumgraphene oxideGraphitePolymersBrain organoidsElectrospun nanofibersLight stimulationReduced graphene oxide (rGO)

Identifiers

PMID40435813
PMCPMC12893844

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.