Evidence map›Paper›PMID 40435438›Full record

ArticlePLoS neglected tropical diseases2025

Calcium-dependent protein kinases 2A involved in the growth of both asexual and sexual stages of Cryptosporidium parvum.

Fanfan Shu, Yujin Huang, Fuxian Yang, Yaqiong Guo, Rui Xu, Lihua Xiao, Yaoyu Feng, Na Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS neglected tropical diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Fanfan ShuState Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, South China Agricultural University, Guangzhou, Guangdong, China.
Yujin HuangState Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, South China Agricultural University, Guangzhou, Guangdong, China.
Fuxian YangState Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, South China Agricultural University, Guangzhou, Guangdong, China.
Yaqiong GuoState Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, South China Agricultural University, Guangzhou, Guangdong, China.
Rui XuState Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, South China Agricultural University, Guangzhou, Guangdong, China.
Lihua XiaoState Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, South China Agricultural University, Guangzhou, Guangdong, China.
Yaoyu FengState Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, South China Agricultural University, Guangzhou, Guangdong, China.
Na LiState Key Laboratory for Animal Disease Control and Prevention, Center for Emerging and Zoonotic Diseases, South China Agricultural University, Guangzhou, Guangdong, China.ORCID 0000-0003-0114-4877

Funding

Double First-Class Discipline Promotion ProjectGuangdong Basic and Applied Basic Research FoundationNational Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaThe Yunnan Key Laboratory of Veterinary Etiological Biology
6 · The paper itself

Abstract

backgroundCryptosporidium parvum is a protozoan pathogen that causes moderate to severe diarrhea in both humans and animals. Calcium-dependent protein kinases (CDPKs) are attractive drug targets against cryptosporidiosis given their critical role in the life cycle of Cryptosporidium spp. and their absence in human and animal hosts. METHODOLOGY/PRINCIPAL

findingsWe used CRISPR-Cas9 technology to endogenously tag the CpCDPK2A gene in C. parvum IIdA20G1-HLJ strain with the hemagglutinin (HA) epitope and to delete the CpCDPK2A gene. An immunofluorescence assay was performed to localize the CpCDPK2A expression in the tagged strain and a luciferase assay was performed to compare growth rates of the tagged and deletion strains in vitro. Oocyst shedding, parasite load, villus length/crypt height ratio and survival of infected mice were used to evaluate the function of CpCDPK2A in vivo. The results revealed that CpCDPK2A was expressed in all the intracellular developmental stages, especially in the motile stages of sporozoites and merozoites. While CpCDPK2A is dispensable, deletion of the gene significantly reduced the growth of late asexual and sexual stages in vitro. In an interferon-γ knockout mouse model, gene deletion of CpCDPK2A reduced oocyst shedding by 25-fold and increased survival of infected mice. CONCLUSIONS/SIGNIFICANCE: These observations suggest that CpCDPK2A may contribute to both asexual and sexual replication of C. parvum and may be a potential target to block the transmission of this important zoonotic pathogen.

Indexed as

Cryptosporidium parvumProtein KinasesProtozoan ProteinsAnimalsCalcium-Calmodulin-Dependent Protein KinasesCRISPR-Cas SystemsCryptosporidiosisFemaleHumansLife Cycle StagesMiceOocystsSporozoitesCalcium-Calmodulin-Dependent Protein KinasesProtein KinasesProtozoan Proteins

Identifiers

PMID40435438
PMCPMC12119106

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.