ArticleFrontiers in cellular and infection microbiology2025
Visual detection of Coxsackievirus A6 using a reverse transcription polymerase spiral reaction method.
Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coxsackievirus A6 (CVA6) ranks as a primary enterovirus associated with hand-foot-mouth disease (HFMD) and herpangina (HA). Given its significant role in these diseases, there is an urgent need for an efficient identification method. This study presents a novel visual approach based on the reverse transcription polymerase spiral reaction (RT-PSR) for the rapid detection of CVA6. We designed an RT-PSR assay that targets and amplifies a segment of the VP1 gene. Hydroxy naphthol blue (HNB) is incorporated as the detection agent in this assay. To evaluate the performance of the RT-PSR assay, we analyzed 142 clinical throat swab samples. The results were benchmarked against those obtained using quantitative reverse transcription - polymerase chain reaction (qRT - PCR). The RT-PSR assay operates at 65°C for 60 minutes and exhibits a detection limit of 10 copies/μL. When tested against other viruses, it consistently yielded negative results, demonstrating its high specificity. Moreover, the RT - PSR assay showed excellent agreement with a commercial qRT - PCR kit. In conclusion, by using HNB as an indicator, the RT - PSR assay emerges as a straightforward and highly sensitive method for detecting CVA6 in symptomatic throat samples. This approach holds great potential for improving the diagnosis and surveillance of CVA6 - related diseases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.