Evidence map›Paper›PMID 40433590›Full record

ArticleFrontiers in molecular biosciences2025

Thioflavin T in-gel staining for

Joseph Oldam, Irina Tchernyshyov, Jennifer Van Eyk, Juan Troncoso, Charles G Glabe, Giulio Agnetti

Abstract read
In one paragraph

Article in Frontiers in molecular biosciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Joseph OldamJohns Hopkins University School of Medicine, Baltimore, MD, United States.
Irina TchernyshyovAdvanced Clinical Biosystems Institute, Cedars-Sinai Hospital, Beverly Hills, CA, United States.
Jennifer Van EykAdvanced Clinical Biosystems Institute, Cedars-Sinai Hospital, Beverly Hills, CA, United States.
Juan TroncosoJohns Hopkins University School of Medicine, Baltimore, MD, United States.
Charles G GlabeUniversity of California Irvine School of Biological Sciences, Irvine, CA, United States.
Giulio AgnettiJohns Hopkins University School of Medicine, Baltimore, MD, United States.

Funding

Research Education ComponentP30AG066507 · NIA · JOHNS HOPKINS UNIVERSITY · PI MARILYN S. ALBERT · 2020 to 2026
$29.3M
NIA NIH HHS P30 AG066507
6 · The paper itself

Abstract

There are limited options to quantify and characterize amyloid species from biological samples in a simple manner. Thioflavin T (ThT) has been used for decades to stain amyloid fibrils, but to our knowledge, we were the first to use it in-gel. Thioflavin T in-gel staining is convenient as it is fast, inexpensive, accessible to most laboratories, and compatible with other fluorescent stains and downstream analyses such as mass spectrometry (MS).

Indexed as

amyloiddesminheart failuremass spectrometryproteinopathies

Identifiers

PMID40433590
PMCPMC12106040

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.