ArticleFrontiers in bioinformatics2025
Exploiting subtractive genomics to identify novel drug targets and new immunogenic candidates against
Article in Frontiers in bioinformatics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- The Homoserine DehydrogenaseMicroorganisms · 2026Article
- Article
- Identification and efficacy assessment of novel immunoprotective antigens from Mycoplasma synoviae for vaccine development.NPJ vaccines · 2025Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Background: Methods: Identification of novel drug targets and the detection of immunogenic factors as potential vaccine candidates were performed. Cytoplasmic proteins were evaluated for their similarity to the human proteome, metabolic pathways, and gut microbiota. On the other hand, surface-exposed proteins were evaluated as immunogenic targets using a reverse vaccinology approach. A multi-epitope vaccine (MEV) was designed based on the immunogenic linear B-cell epitopes of three autotransporters and the beta domain of SphB2 as a scaffold for MEV. Molecular docking, immune simulation results, and molecular dynamics simulations were performed to evaluate the binding affinity and feasibility of interaction between chimeric MEVs and immune receptors. Results: Six proteins were identified as excellent potential drug targets, including elongation factor P (WP_003810194.1), Aspartate kinase (WP_010930633.1), 50S ribosomal protein L21 (WP_003807462.1), Homoserine dehydrogenase (WP_003813074.1), Carboxynorspermidine decarboxylase (WP_003814461.1), and PTS sugar transporter subunit IIA (WP_010929966.1). On the other hand, reverse vaccinology identified nine immunogenic proteins, including BapA (WP_010930805.1), BrkA (WP_010931506.1), SphB2 (WP_041166323.1), TcfA (WP_010930243.1), FliK (WP_041166144.1), Fimbrial protein (WP_010930199.1), TolA (WP_010931418.1), DD-metalloendopeptidase (WP_003811022.1), and an I78 family peptidase inhibitor protein (WP_003812179.1). SphB2-based MEV was designed using six linear B-cell epitopes of the extracellular loops of the autotransporters. The binding affinity and feasibility of the interaction between MEV and TLR2, TLR4, and HLA-DR-B were computationally confirmed by molecular dynamics. Conclusion: It appears that proteins involved in translation and metabolism can be considered novel drug targets. Furthermore, this study highlights autotransporter proteins as promising immune targets. There is no doubt that experimental work should be conducted to confirm the results in the future.
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