Evidence map›Paper›PMID 40431747›Full record

ArticleViruses2025

Epidemiological and Molecular Surveillance of Aichi Virus A at Different Stages of Sewage Treatment: A One-Year Study in the Southeast of Brazil.

Mariah C A do Nascimento, Camila R Rosa, Meriane Demoliner, Dayla B Geraldini, Guilherme R F Campos, Daniela M Quevedo, Rafael N Miceli, Fernando R Spilki, João Pessoa Araújo, Marilia F Calmon and 1 more

Abstract read
In one paragraph

Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Wastewater Surveillance of Aichi Virus in Baltimore.Pathogens (Basel, Switzerland) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Mariah C A do NascimentoDepartment of Biology, São Paulo State University (UNESP), São José do Rio Preto 15054-000, SP, Brazil.ORCID 0000-0002-6422-4068
Camila R RosaDepartment of Biology, São Paulo State University (UNESP), São José do Rio Preto 15054-000, SP, Brazil.
Meriane DemolinerMolecular Microbiology Laboratory, University Feevale, Novo Hamburgo 93525-075, RS, Brazil.ORCID 0000-0002-7160-7649
Dayla B GeraldiniDepartment of Biology, São Paulo State University (UNESP), São José do Rio Preto 15054-000, SP, Brazil.
Guilherme R F CamposDepartment of Dermatological, Infectious and Parasitic Diseases, Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, SP, Brazil.ORCID 0000-0003-1118-8486
Daniela M QuevedoInstitute of Exact and Technological Sciences (ICET), University Feevale, Novo Hamburgo 93525-075, RS, Brazil.ORCID 0000-0003-2169-9781
Rafael N MiceliSeMAE-Autonomous Municipal Water and Sewage Service, São José do Rio Preto 15048-000, SP, Brazil.
Fernando R SpilkiMolecular Microbiology Laboratory, University Feevale, Novo Hamburgo 93525-075, RS, Brazil.ORCID 0000-0001-5804-7045
João Pessoa AraújoBiotechnology Institute, São Paulo State University (UNESP), Botucatu 18618-687, SP, Brazil.ORCID 0000-0002-9153-1485
Marilia F CalmonDepartment of Biology, São Paulo State University (UNESP), São José do Rio Preto 15054-000, SP, Brazil.ORCID 0000-0001-5203-0103
Paula RahalDepartment of Biology, São Paulo State University (UNESP), São José do Rio Preto 15054-000, SP, Brazil.ORCID 0000-0001-5693-6148

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior Financial code 001Fundação de Amparo à Pesquisa do Estado de São Paulo 2023/01598-9Fundação de Amparo à Pesquisa do Estado de São Paulo 2024/09651-9Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul 23/2551-0002221-4National Council for Scientific and Technological Development 405786/2022-0
6 · The paper itself

Abstract

Enteric viruses, such as the Aichi virus (AiV), pose a potential health risk due to their high excretion rates through fecal elimination, limited removal during treatment processes, and prolonged survival, highlighting the need to assess the potential for exposure and disease transmission through sanitation systems. This study investigated the prevalence of AiV at three key stages of sewage treatment in the city of São José do Rio Preto, São Paulo state, Brazil, as well as its viral concentrations, infectious potential, and molecular characterization. The data were also analyzed for potential correlations with reported diarrheal disease cases in the city and the physicochemical properties of sewage. The methodology employed included Nested PCR, qPCR, Sanger Sequencing, and phylogenetic analysis, as well as infectivity testing in cell cultures. The prevalence of AiV throughout the year in raw sewage samples was 90.4%, 78.8% in post-anaerobic biological treatment, and 71.1% in post-chemical treatment, totaling 125 positive samples out of 156, being characterized as AiV genotype A. The virus also demonstrated persistence and infectious potential at all three stages analyzed. The AiV-A mean concentration ranged from 2.05 log

Indexed as

KobuvirusPicornaviridae InfectionsSewageBrazilDiarrheaEpidemiological MonitoringFecesGenotypeHumansPhylogenyPrevalenceSewageenteric virusesinfectivity assaysewage treatment

Identifiers

PMID40431747
PMCPMC12115472

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.