Evidence map›Paper›PMID 40431659›Full record

ArticleViruses2025

Identification of TRIM21 and TRIM14 as Antiviral Factors Against Langat and Zika Viruses.

Pham-Tue-Hung Tran, Mir Himayet Kabir, Naveed Asghar, Matthew R Hathaway, Assim Hayderi, Roger Karlsson, Anders Karlsson, Travis Taylor, Wessam Melik, Magnus Johansson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Pham-Tue-Hung TranSchool of Medical Science, Faculty of Medicine and Health, Örebro University, SE-70362 Örebro, Sweden.ORCID 0000-0002-8366-9310
Mir Himayet KabirDepartment of Medical Microbiology and Immunology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43612, USA.
Naveed AsgharSchool of Medical Science, Faculty of Medicine and Health, Örebro University, SE-70362 Örebro, Sweden.ORCID 0000-0003-4442-8503
Matthew R HathawayDepartment of Medical Microbiology and Immunology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43612, USA.
Assim HayderiSchool of Medical Science, Faculty of Medicine and Health, Örebro University, SE-70362 Örebro, Sweden.
Roger KarlssonDepartment of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy of the University of Gothenburg, SE-40234 Gothenburg, Sweden.ORCID 0000-0002-5919-2639
Anders KarlssonNanoxis Consulting AB, SE-40016 Gothenburg, Sweden.ORCID 0000-0002-2927-481X
Travis TaylorDepartment of Medical Microbiology and Immunology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43612, USA.ORCID 0000-0002-3819-8548
Wessam MelikSchool of Medical Science, Faculty of Medicine and Health, Örebro University, SE-70362 Örebro, Sweden.ORCID 0000-0001-9876-6239
Magnus JohanssonSchool of Medical Science, Faculty of Medicine and Health, Örebro University, SE-70362 Örebro, Sweden.ORCID 0000-0003-3962-2141

Funding

Understanding the proviral role for TRAF6 interaction with the viral protease in flavivirus replication and pathogenesisR01AI153496 · NIAID · UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS · PI TAYLOR, ROGER TRAVIS · 2020 to 2024
$1.9M
NIAID NIH HHS R01 AI153496The Knowledge foundation 20200063 and 20210052
6 · The paper itself

Abstract

Flaviviruses are usually transmitted to humans via mosquito or tick bites, whose infections may lead to severe diseases and fatality. During intracellular infection, they remodel the endoplasmic reticulum (ER) membrane to generate compartments scaffolding the replication complex (RC) where replication of the viral genome takes place. In this study, we purified the ER membrane fraction of virus infected cells to identify the proteins that were enriched during flavivirus infection. We found that tripartite motif-containing proteins (TRIMs) including TRIM38, TRIM21, and TRIM14 were significantly enriched during infection with mosquito-borne (West Nile virus strain Kunjin and Zika virus (ZIKV)) and tick-borne (Langat virus (LGTV)) flaviviruses. Further characterizations showed that TRIM21 and TRIM14 act as restriction factors against ZIKV and LGTV, while TRIM38 hinders ZIKV infection. These TRIMs worked as interferon-stimulated genes to mediate IFN-I response against LGTV and ZIKV infections. Restriction of ZIKV by TRIM14 and TRIM38 coincides with their colocalization with ZIKV NS3. TRIM14-mediated LGTV restriction coincides with its colocalization with LGTV NS3 and NS5 proteins. However, TRIM21 did not colocalize with ZIKV and LGTV NS3 or NS5 protein suggesting its antiviral activity is not dependent on direct targeting the viral enzyme. Finally, we demonstrated that overexpression of TRIM21 and TRIM14 restricted LGTV replication.

Indexed as

FlavivirusRibonucleoproteinsTripartite Motif ProteinsZika VirusAnimalsCell LineEndoplasmic ReticulumHEK293 CellsHumansIntracellular Signaling Peptides and ProteinsSS-A AntigenVirus ReplicationWest Nile virusZika Virus InfectionIntracellular Signaling Peptides and ProteinsRibonucleoproteinsSS-A AntigenTRIM14 protein, humanTripartite Motif Proteinsantiviral host factorflavivirusLGTVNS3NS5TRIM14TRIM21TRIM38WNVZIKV

Identifiers

PMID40431659
PMCPMC12116035

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.