ArticleVeterinary sciences2025
Comparative Assessment of Morphometry, Morphology, and Maturation Capacity of Vitrified Cattle Oocytes in Different Media.
Article in Veterinary sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Comparative study onFrontiers in veterinary science · 2025Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to compare the morphometry, morphology, and maturation capacity of cattle oocytes subjected to vitrification using different vitrification and maturation media. In Experiment 1, a total of 900 oocytes were divided into three groups: (1) matured before vitrification, (2) non-vitrified, and (3) vitrified as immature oocytes using the straw vitrification method. Morphometric parameters, including oocyte diameter, ooplasm, zona pellucida width (ZPW), granulosa cell width (GRSW), and zona pellucida-granulosa cell width (ZP GRSW), were measured (µm) before and after cryopreservation. In Experiment 2, the maturation capacity of three in vitro maturation (IVM) media (VitroMat-Protect™, BO-IVM™, and TCM199) was evaluated based on cumulus-oocyte complex (COC) expansion and polar body (PB) extrusion. Morphological abnormalities such as fragmented polar bodies (FPBs), large vacuoles (LVs), degenerated oocytes (DOs), and cracked cytoplasm (CC) were recorded. While vitrification did not significantly affect the oocyte diameter, ooplasm, or ZPW, it significantly reduced the GRSW and ZP GRSW. BO-IVM™ supported the highest COC expansion rate, while TCM199 had the lowest. Among vitrified oocytes, the highest PB extrusion rates were observed in BO-IVM
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