Evidence map›Paper›PMID 40431235›Full record

ReviewMicroorganisms2025

New Methodologies as Opportunities in the Study of Bacterial Biofilms, Including Food-Related Applications.

Francesca Coppola, Florinda Fratianni, Vittorio Bianco, Zhe Wang, Michela Pellegrini, Raffaele Coppola, Filomena Nazzaro

Abstract readReview
In one paragraph

Review in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Microorganisms · 2025
    Review
  12. Review
  13. Review
  14. Review
  15. Review
  16. Towards EffectiveInternational journal of molecular sciences · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Francesca CoppolaInstitute of Food Science, CNR-ISA, 83100 Avellino, Italy.ORCID 0000-0002-1620-6818
Florinda FratianniInstitute of Food Science, CNR-ISA, 83100 Avellino, Italy.ORCID 0000-0001-5368-570X
Vittorio BiancoInstitute of Applied Sciences and Intelligent Systems "Eduardo Caianiello", Via Campi Flegrei, 80078 Pozzuoli, Italy.
Zhe WangInstitute of Applied Sciences and Intelligent Systems "Eduardo Caianiello", Via Campi Flegrei, 80078 Pozzuoli, Italy.
Michela PellegriniDepartment of Agricultural, Food, Environmental and Animal Science, University of Udine, Via Sondrio 2/a, 33100 Udine, Italy.
Raffaele CoppolaInstitute of Food Science, CNR-ISA, 83100 Avellino, Italy.ORCID 0000-0003-1849-6801
Filomena NazzaroInstitute of Food Science, CNR-ISA, 83100 Avellino, Italy.ORCID 0000-0002-5759-7499

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Traditional food technologies, while essential, often face limitations in sensitivity, real-time detection, and adaptability to complex biological systems such as microbial biofilms. These constraints have created a growing demand for more advanced, precise, and non-invasive tools to ensure food safety and quality. In response to these challenges, cross-disciplinary technological integration has opened new opportunities for the food industry and public health, leveraging methods originally developed in other scientific fields. Although their industrial-scale implementation is still evolving, their application in research and pilot settings has already significantly improved our ability to detect and control biofilms, thereby strengthening food safety protocols. Advanced analytical techniques, the identification of pathogenic species and their virulence markers, and the screening of "natural" antimicrobial compounds can now be conceptualized as interconnected elements within a virtual framework centered on "food" and "biofilm". In this short review, starting from the basic concepts of biofilm and associated microorganisms, we highlight a selection of emerging analytical approaches-from optical methods, microfluidics, Atomic Force Microscopy (AFM), and biospeckle techniques to molecular strategies like CRISPR, qPCR, and NGS, and the use of organoids. Initially conceived for biomedical and biotechnological applications, these tools have recently demonstrated their value in food science by enhancing our understanding of biofilm behavior and supporting the discovery of novel anti-biofilm strategies.

Indexed as

biofilmbiospeckleCRISPRDNA-methodsmicrofluidicsmicroscopyorganoids

Identifiers

PMID40431235
PMCPMC12114119

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.