Cryo-Electron Microscopy of BfpB Reveals a Type IVb Secretin Multimer Adapted to Accommodate the Exceptionally Wide Bundle-Forming Pilus.

Janay I Little, Pradip Kumar Singh, Montserrat Samsó, Michael S Donnenberg

Abstract read

In one paragraph

Article in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

4 authors.

Janay I LittleSchool of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA.ORCID 0000-0002-7174-5742

Pradip Kumar SinghSchool of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA.

Montserrat SamsóSchool of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA.ORCID 0000-0002-2788-3283

Michael S DonnenbergSchool of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA.ORCID 0000-0002-8818-3184

Funding

NIH HHS 1R21AI123964-01A1

6 · The paper itself

Abstract

Type IV pili (T4Ps) are multifunctional surface fibers essential for bacterial motility, adhesion, and virulence, found across Gram-negative and Gram-positive bacteria and archaea. Detailed descriptions of T4P structural biology are allowing progress in understanding T4P biogenesis. Secretins, large outer membrane channels, are crucial for T4P extrusion in Gram-negative bacteria. Using cryo-EM and AlphaFold, we modeled the structure of BfpB, the secretin of the Bundle-Forming Pilus (BFP) of enteropathogenic

Indexed as

Enteropathogenic Escherichia coliEscherichia coli ProteinsFimbriae, BacterialFimbriae ProteinsSecretinCryoelectron MicroscopyModels, MolecularProtein ConformationProtein MultimerizationEscherichia coli ProteinsFimbriae ProteinsSecretinAlphaFoldcryo-EMprotein structuresecretinstype IV pili

Identifiers

PMID40430791

DOI10.3390/pathogens14050471

PMCPMC12114550

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.