ArticleLife (Basel, Switzerland)2025
Microfluidic Sorting Can Be Applied for Assisted Reproduction Sperm Selection in Different Cases of Semen Abnormalities.
Article in Life (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Comparative analysis of sperm preparation techniques on DNA fragmentation and clinical outcomes: a network meta-analysis.Frontiers in endocrinology · 2026Pooled it
- Enhancement of Y-bearing sperm enrichment in swamp buffalo using a TLR7/8 agonist (R848) combined with discontinuous Percoll density gradient centrifugation.Veterinary world · 2026Article
- Does Microfluidic Sperm Sorting Affect Embryo Ploidy in Human In Vitro Fertilization?Bulletin of experimental biology and medicine · 2026Article
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Authors and funding
8 authors.
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Abstract
Sperm preparation is a critical step in assisted reproduction, aiming to isolate spermatozoa with optimal characteristics and high fertilizing potential. Traditional sperm selection methods involve centrifugation, which may cause sperm damage. Microfluidic sperm sorting (MSS) offers an alternative approach, mimicking the female reproductive tract environment, avoiding centrifugation, and reducing manipulation and processing time. This study aims to compare the performance of MSS and Swim-up (SU) in 26 normozoospermic, 31 hyperviscous normozoospermic, 15 oligozoospermic, and 9 asthenozoospermic subjects. Semen samples were collected from male subjects undergoing routine semen analysis at Careggi University Hospital, Florence. Sperm selection was carried out using both SU and MSS. The parameters assessed included sperm motility, viability, concentration, kinematics, DNA fragmentation (sDF), chromatin compaction, and oxidative status. Both SU and MSS improved sperm characteristics compared to unselected samples. MSS isolated high-quality spermatozoa with lower sDF and higher chromatin compaction than SU, not only in normozoospermic samples but also in samples with semen defects like hyperviscosity, low concentration and/or motility, and high sDF. In conclusion, the use of microfluidics may enhance the chances of successful fertilization and improve reproductive outcomes, especially for individuals with compromised semen quality where conventional methods may fail.
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