Evidence map›Paper›PMID 40429826›Full record

ArticleInternational journal of molecular sciences2025

Benzimidazole-Derived B2 as a Fluorescent Probe for Bacterial Outer Membrane Vesicle (OMV) Labeling: Integrating DFT, Molecular Dynamics, Flow Cytometry, and Confocal Microscopy.

Francisco Parra, Alexander Carreño, Evys Ancede-Gallardo, Diana Majluf, Jorge A Soto, Romina V Sepúlveda, Daniel Aguayo, María Carolina Otero, Iván L Calderón, Fernando Gil and 1 more

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Francisco ParraLaboratorio de Genética y Patogénesis Bacteriana, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370186, Chile.ORCID 0009-0004-1541-8968
Alexander CarreñoLaboratory of Organometallic Synthesis, Departamento de Ciencias Químicas, Facultad de Ciencias Exactas, Universidad Andres Bello, Santiago 8370186, Chile.ORCID 0000-0001-9733-8224
Evys Ancede-GallardoLaboratory of Organometallic Synthesis, Departamento de Ciencias Químicas, Facultad de Ciencias Exactas, Universidad Andres Bello, Santiago 8370186, Chile.ORCID 0000-0002-3478-6035
Diana MajlufLaboratorio de Genética y Patogénesis Bacteriana, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370186, Chile.
Jorge A SotoMillennium Institute on Immunology and Immunotherapy, Laboratorio de Inmunología Traslacional, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370186, Chile.ORCID 0000-0003-0335-9759
Romina V SepúlvedaCenter for Bioinformatics and Integrative Biology (CBIB), Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370146, Chile.ORCID 0000-0001-8899-1440
Daniel AguayoInstituto de Tecnología para la Innovación en Salud y Bienestar (ITISB), Facultad de Ingeniería, Universidad Andres Bello, Viña del Mar 2531015, Chile.ORCID 0000-0001-6807-0820
María Carolina OteroEscuela de Química y Farmacia, Facultad de Medicina, Universidad Andres Bello, Santiago 7591538, Chile.ORCID 0000-0002-8608-9813
Iván L CalderónLaboratorio de RNAs Bacterianos, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370186, Chile.ORCID 0000-0003-3554-0894
Fernando GilSchool of Medicine, Faculty of Medicine, Universidad de los Andes, Santiago 7620001, Chile.ORCID 0009-0006-1666-7987
Juan A FuentesLaboratorio de Genética y Patogénesis Bacteriana, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370186, Chile.ORCID 0000-0002-9374-0707

Funding

ANID-Millennium Science Initiative Program NCN2024_047FONDECYT 11251048FONDECYT 1171654FONDECYT 1220584FONDECYT 1230917FONDECYT 1231866PAI project SA77210051
6 · The paper itself

Abstract

Bacterial outer membrane vesicles (OMVs) are nanoscale extracellular structures produced by Gram-negative bacteria that are critical for microbial biology and host-pathogen interactions and have great potential in biotechnological applications. Despite the availability of fluorescent dyes for OMV studies, many are repurposed from eukaryotic extracellular vesicle research and are not explicitly optimized for OMVs, leading to challenges in achieving consistent labeling, minimizing background noise, and preserving vesicle integrity during analyses. This study evaluates B2, a benzimidazole-derived fluorophore, for OMV labeling in advanced techniques like flow cytometry and confocal microscopy. OMVs were isolated from

Indexed as

Bacterial Outer MembraneBenzimidazolesFluorescent DyesEscherichia coliFlow CytometryHT29 CellsHumansMicroscopy, ConfocalMolecular Dynamics SimulationbenzimidazoleBenzimidazolesFluorescent Dyesbenzimidazole compound B2confocal microscopyflow cytometryfluorescent dyesfluorescent labeling of OMVsintramolecular hydrogen bondOMVsouter membrane vesicles

Identifiers

PMID40429826
PMCPMC12112470

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.