Evidence map›Paper›PMID 40428351›Full record

ArticleGenes2025

Development and Application of a TaqMan-Based qPCR Assay for Detecting ENTV-2 in Goats.

Pengfei Li, Haike Yin, Xiaoan Cao, Xi Lan, Jinyan Wu, Jijun He, Ligang Yuan, Youjun Shang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Pengfei LiState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Haike YinBaoji Animal Husbandry and Veterinary Center, Baoji 721001, China.
Xiaoan CaoState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Xi LanState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Jinyan WuState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Jijun HeState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Ligang YuanCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.ORCID 0000-0002-8386-0262
Youjun ShangState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.ORCID 0000-0001-8016-0511

Funding

Ligang Yuan GSAU-JSFW-2023-07, GSAU-JSFW-2023-08Youjun Shang 2022YFD1602203Youjun Shang 2024BBF02021Youjun Shang 22CX8NA012Youjun Shang CARS-39-04B
6 · The paper itself

Abstract

backgroundIn recent years, enzootic nasal tumor virus 2 (ENTV-2) has become prevalent in China, resulting in substantial economic losses for the goat industry. In order to enrich the availability of detection methods for ENTV-2, this study developed an expedited and accurate reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) assay to facilitate the detection and quantification of ENTV-2.

methodsSpecifically, a pair of primers and a TaqMan probe targeting conserved regions of the

resultsBoth methods exhibited a detection limit for the ENTV-2 standard plasmid at 10

conclusionsIn conclusion, both methods are highly specific for the

Indexed as

Goat DiseasesReal-Time Polymerase Chain ReactionTumor Virus InfectionsAnimalsChinaGoatsRNA, ViralRNA, Viralenzootic nasal adenocarcinomaenzootic nasal tumor virus 2pro geneRT-qPCR

Identifiers

PMID40428351
PMCPMC12111429

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.