Optimizing Rhamnolipid Performance by Modulating the Expression of Fatty Acid Synthesis Genes

Junpeng Lu, Zhenhua Chen, Huiming Zhu, Qinghai Tang, Zhili Yang

Abstract read

In one paragraph

Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Junpeng LuSystems Biology Laboratory, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.

Zhenhua ChenSystems Biology Laboratory, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.

Huiming ZhuSystems Biology Laboratory, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.

Qinghai TangSystems Biology Laboratory, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.

Zhili YangSystems Biology Laboratory, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.ORCID 0000-0002-0885-4575

Funding

Zhejiang Provincial Natural Science Foundation of China LQ24C010003

6 · The paper itself

Abstract

BACKGROUND/

objectivesRhamnolipids (RLs) are biosurfactants with significant industrial and environmental potential, which physicochemical properties depend greatly on their fatty acyl chain composition. This study investigated the impact of genetically modulating the fatty acid synthesis genes METHODS AND

resultsUsing temperature-sensitive mutants and suppressor strains for these essential genes, we successfully engineered RLs with altered fatty acyl chain lengths and saturation levels. LC-MS/MS analyses showed that deletion and overexpression of

conclusionsThese findings demonstrate the feasibility of optimizing RL properties through targeted genetic manipulation, offering valuable insights for designing customized biosurfactants for diverse industrial and environmental applications.

Indexed as

Bacterial ProteinsFatty AcidsFatty Acid Synthase, Type IIGlycolipidsPseudomonas aeruginosaGene Expression Regulation, BacterialSurface-Active AgentsBacterial ProteinsFatty AcidsFatty Acid Synthase, Type IIGlycolipidsrhamnolipidSurface-Active Agentsbiosurfactantfatty acyl chainPseudomonas aeruginosarhamnolipids

Identifiers

PMID40428336

DOI10.3390/genes16050515

PMCPMC12111694

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