Evidence map›Paper›PMID 40427674›Full record

ArticleBiology2025

Construction and Influence of Induced Pluripotent Stem Cells on Early Embryo Development in Black Bone Sheep.

Daqing Wang, Yiyi Liu, Lu Li, Xin Li, Xin Cheng, Zhihui Guo, Guifang Cao, Yong Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Daqing WangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Yiyi LiuCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Lu LiCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Xin LiCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Xin ChengCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Zhihui GuoCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Guifang CaoCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.
Yong ZhangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China.

Funding

Creation of new materials for cattle breeding by gene editing for Heat stress Resistance" (No. 2023ZD04050). No. 2023ZD04050
6 · The paper itself

Abstract

The piggyBac+TET-on transposon induction system has a high efficiency in integrating exogenous genes in multiple cell types, can precisely integrate to reduce genomic damage, has a flexible gene expression regulation, and a strong genetic stability. When used in conjunction with somatic cell nuclear transfer experiments, it can precisely and effectively reveal the intrinsic mechanisms of early biological development. This study successfully reprogrammed black-boned sheep fibroblasts (SFs) into induced pluripotent stem cells (iPSCs) using the piggyBac+TET-on transposon system and investigated their impact on early embryonic development. Seven exogenous reprogramming factors (bovine OCT4, SOX2, KLF4, cMyc, porcine NANOG, Lin-28, and SV40 Large T) were delivered into SFs, successfully inducing iPSCs. A growth performance analysis revealed that iPSC clones exhibited a raised or flat morphology with clear edges, positive alkaline phosphatase staining, and normal karyotypes. The transcriptome analysis indicated a significant enrichment of iPSCs in oxidative phosphorylation and cell proliferation pathways, with an up-regulated expression of the ATP5B, SDHB, Bcl-2, CDK1, and Cyclin D1 genes and a down-regulated expression of BAX (

Indexed as

black bone sheepcellular reprogrammingpiggyBac vectorpluripotent stem cells

Identifiers

PMID40427674
PMCPMC12109116

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.