Evidence map›Paper›PMID 40427530›Full record

ArticleBiomolecules2025

Ngn2-Induced Differentiation of the NG108-15 Cell Line Enhances Motor Neuronal Differentiation and Neuromuscular Junction Formation.

Madeline Meli, Kristy Swiderski, Jinchao Gu, Ben Rollo, Ben Bartlett, Marissa K Caldow, Gordon S Lynch, Patrick Kwan, Huseyin Sumer, Brett A Cromer

Erratum issuedAbstract read
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In one paragraph

Article in Biomolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Madeline MeliDepartment of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Melbourne, VIC 3122, Australia.
Kristy SwiderskiCentre for Muscle Research, Department of Anatomy and Physiology, School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3122, Australia.
Jinchao GuDepartment of Neuroscience, Central Clinical School, Monash University, Alfred Centre, Melbourne, VIC 3122, Australia.ORCID 0009-0008-5982-465X
Ben RolloDepartment of Neuroscience, Central Clinical School, Monash University, Alfred Centre, Melbourne, VIC 3122, Australia.
Ben BartlettDepartment of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Melbourne, VIC 3122, Australia.
Marissa K CaldowCentre for Muscle Research, Department of Anatomy and Physiology, School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3122, Australia.
Gordon S LynchCentre for Muscle Research, Department of Anatomy and Physiology, School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3122, Australia.ORCID 0000-0001-9220-9810
Patrick KwanDepartment of Neuroscience, Central Clinical School, Monash University, Alfred Centre, Melbourne, VIC 3122, Australia.
Huseyin SumerDepartment of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Melbourne, VIC 3122, Australia.ORCID 0000-0001-9413-5579
Brett A CromerDepartment of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Melbourne, VIC 3122, Australia.ORCID 0000-0003-0743-1535

Funding

Medical Research Future Fund Stem Cell Therapies Mission (AU) MRF2015957
6 · The paper itself

Abstract

The neuronal progenitor NG108-15 neuroblastoma x glioma cell line proliferates indefinitely in vitro and is capable of directed differentiation into cholinergic neurons. The cell line is a robust model for investigating neuronal differentiation and function in vitro. The lineage-specific transcription factor-mediated differentiation of pluripotent stem cell lines (PSCs) leads to more rapid, efficient, and functional neurons. In this study, we tested the hypothesis that transcription factors could also drive the fate of an immortalised cell line. We first established a stable NG108-15 cell line, by piggyBac (pBac) transposition, that conditionally expresses neurogenin-2 (Ngn2), a common transcription factor for specifying neuronal fate. Following doxycycline-induction of Ngn2, we observed more rapid and efficient differentiation, and improved neurite outgrowth and viability compared with the WT cell line. Moreover, when co-cultured with C2C12 mouse myotubes, the modified NG108-15 cells resulted in significantly larger acetylcholine receptor (AChR) aggregates, suggesting enhanced neuromuscular junction (NMJ) formation. These findings describe a novel methodology for differentiating NG108-15 cells more efficiently, to enhance the usefulness of the cell line as a motor neuron model.

Indexed as

Basic Helix-Loop-Helix ProteinsCell DifferentiationMotor NeuronsNerve Tissue ProteinsNeuromuscular JunctionAnimalsCell Line, TumorHumansMiceMuscle Fibers, SkeletalReceptors, CholinergicBasic Helix-Loop-Helix ProteinsNerve Tissue ProteinsNeurog2 protein, mouseReceptors, Cholinergiccell differentiationcell linemotor neuronsmuscle fibresmyogenesisneuroblastomaneuromuscular junctionneuronsskeletal muscletranscription factors

Identifiers

PMID40427530
PMCPMC12108711

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.