Evidence map›Paper›PMID 40427246›Full record

ArticleAnimals : an open access journal from MDPI2025

Innovative Approaches to Avoid Antibiotic Use in Equine Semen Cryopreservation: Advancing Sustainable Reproductive Technologies.

Sonsoles Mercedes Zabala, Consuelo Serres, Natalia Montero, Francisco Crespo, Pedro Luis Lorenzo, Verónica Pérez-Aguilera, Agustín Oliet, Virginia Hijón, Santiago Moreno, Bruno González-Zorn and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Sonsoles Mercedes ZabalaAnimal Medicine and Surgery Department, Veterinary Faculty, Complutense University of Madrid (UCM), Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.ORCID 0009-0005-6595-8871
Consuelo SerresAnimal Medicine and Surgery Department, Veterinary Faculty, Complutense University of Madrid (UCM), Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.ORCID 0000-0002-6713-5124
Natalia MonteroAnimal Health Department, Veterinary Faculty, Complutense University of Madrid (UCM), Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
Francisco CrespoAnimal Medicine and Surgery Department, Veterinary Faculty, Complutense University of Madrid (UCM), Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.ORCID 0000-0002-6946-3527
Pedro Luis LorenzoPhysiology Department, Veterinary Faculty, Complutense University of Madrid (UCM), Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
Verónica Pérez-AguileraCentro Militar de Cría Caballar de Ávila (CCFAA), C/Arsenio Gutiérrez Palacios s/n, 05005 Ávila, Spain.ORCID 0009-0003-3404-2137
Agustín OlietAnimal Selection and Reproduction Center, Madrid Institute for Rural, Agricultural and Food Research and Development (IMIDRA), Ctra. Colmenar Viejo a Guadalix de la Sierra, km 1, Colmenar Viejo, 28770 Madrid, Spain.ORCID 0000-0002-0307-1256
Virginia HijónAnimal Selection and Reproduction Center, Madrid Institute for Rural, Agricultural and Food Research and Development (IMIDRA), Ctra. Colmenar Viejo a Guadalix de la Sierra, km 1, Colmenar Viejo, 28770 Madrid, Spain.
Santiago MorenoAnimal Selection and Reproduction Center, Madrid Institute for Rural, Agricultural and Food Research and Development (IMIDRA), Ctra. Colmenar Viejo a Guadalix de la Sierra, km 1, Colmenar Viejo, 28770 Madrid, Spain.
Bruno González-ZornAnimal Health Department, Veterinary Faculty, Complutense University of Madrid (UCM), Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
Luna Gutiérrez-CepedaAnimal Medicine and Surgery Department, Veterinary Faculty, Complutense University of Madrid (UCM), Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.ORCID 0000-0001-5238-3590

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study evaluated the impact of different processing techniques on microbial load and sperm quality in frozen-thawed equine semen to identify alternatives to reduce the preventive use of antibiotics. Semen was obtained and processed under rigorous hygiene measures from ten stallions, using four protocols: Simple Centrifugation with antibiotics (S+) and Simple Centrifugation (S-), Filtration (F-) and Single-Layer Colloidal Centrifugation (C-) in an antibiotic-free extender. Microbial load in different culture media, sperm viability and motility were assessed. Microbial load results were consistent across protocols, except in Columbia 5% Sheep Blood Agar media, where S- exhibited higher microbial load than S+ (

Indexed as

absence of antibioticsantibiotic-free extenderEquipure®filtrationfrozen equine semensemen microbial loadsimple centrifugationsingle-layer colloidal centrifugationSpermFilter®

Identifiers

PMID40427246
PMCPMC12108249

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.