Valorisation of insect infested sweet sorghum reeds towards production of a fermented beverage.

Sweet sorghum variety (Sorghum bicolour (L)) commonly known as sweet reeds, Ntšhe, in Setswana, is a valuable cash crop mostly for small scale farmers in Botswana and other southern African countries. These reeds are widely consumed as a delicacy and contribute significantly to food security, employment, and rural incomes. However, infestations by the larval stages of Chilo partellus (stem borer moths) lead to substantial economic losses, as consumers reject worm-infested reeds. To mitigate these losses, valorisation of condemned sweet reeds is attractive. Here, we took advantage of our understanding of yeast-insect interactions to isolate yeasts associated with larval stages of the stem borer moths and investigated their potential for use in the production of an alcoholic sweet sorghum beverage. We report the isolation of thirty-two yeast strains from the larvae and assessed their ability to ferment the simplest sugar, glucose, a constituent of the sweet sorghum juice. Out of the selected yeasts, a subset of fourteen strains belonging to Hanseniaspora and Candida genera were further characterised based on their capacity to ferment more sugars found in sweet sorghum juice. We further assessed the isolates for the ability to tolerate brewing/fermentation-associated stresses and production of complex aroma profiles towards the use of sweet sorghum juice as a sole feedstock to produce a commercial beverage. Our findings suggest that yeast-insect interactions offer a promising approach for converting rejected sweet sorghum stalks into a novel alcoholic beverage, adding economic value to an otherwise discarded resource. Clinical trial number Not applicable.