Evidence map›Paper›PMID 40426039›Full record

ArticleBMC genomics2025

A tritrophic plant-insect-pathogen system used to develop a closely linked Rag2 and Rsv1-h recombinant haplotype in double-resistant soybean germplasm.

Luis G Posadas, Edson Ll Baldin, Lia Marchi-Werle, Tiffany M Heng-Moss, Scott Speck, Robert M Stupar, Kent M Eskridge, George L Graef

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Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Luis G PosadasDepartment of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Edson Ll BaldinDepartment of Crop Protection, College of Agronomic Sciences, São Paulo State University, Botucatu, São Paulo, 18610-307, Brazil.
Lia Marchi-WerleExacto®, LLC. , Sharon, WI, 53585, USA.
Tiffany M Heng-MossCollege of Agricultural Sciences and Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Scott SpeckBeck's Hybrids, North Platte, NE, 69101, USA.
Robert M StuparDepartment of Agronomy and Plant Genetics, University of Minnesota, Saint Paul, MN, 55108, USA.
Kent M EskridgeDepartment of Statistics, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
George L GraefDepartment of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA. lposadas2@unl.edu.

Funding

Nebraska Soybean Board Soybean Breeding and Genetics
6 · The paper itself

Abstract

backgroundThe colocalization of two resistance (R) genes on chromosome 13 of soybean (Glycine max (L.) Merrill) that confer resistance against the soybean aphid (Aphis glycines) and soybean mosaic virus (SMV) gives rise to a very unique R-avr tritrophic incompatible interaction system that goes across biological kingdoms. In this tritrophic system, the insect is the only natural vector of the virus and soybean is a host-plant for both pests/pathogen. The almost unavoidable co-evolution of pathogen-vector with that of the R-genes in soybean plants through an endless arms race to avoid each other's defense-attack mechanisms raises interesting questions. The objectives of this work were to (i) develop double-resistant recombinant inbred lines (RILs) with a Rag2-Rsv1-h gene haplotype in coupling phase using resistance alleles from two different genetic sources (PI 243540 (Rag2) and Suweon 97 (Rsv1-h)), (ii) confirm phenotypically the resistant reaction against both pests in double-resistant RILs, and (iii) dissect the Rag2-Rsv1-h region with molecular markers and investigate the potential for structural variation.

resultsWe observed a recombination event in identified double-resistant F

conclusionsTo our knowledge, this is the first report of double-resistant Rag2-Rsv1-h soybean RILs that used a plant-insect-pathogen tritrophic system for germplasm enhancement. The co-occurrence of Rag and Rsv genes in a region that clusters resistance genes on chromosome 13 may be a unique feature of domesticated soybean. The recombinant genotypes will be useful in breeding to develop soybean cultivars with resistance to both the vector and the virus. The parental and recombinant genotypes may be helpful in future studies to elucidate interesting evolutionary questions regarding vector, host, and virus tritrophic systems.

Indexed as

Disease ResistanceGlycine maxHaplotypesPlant DiseasesAnimalsAphidsHost-Pathogen InteractionsPotyvirusRecombination, GeneticBreedingCandidate genesComparative genomic hybridizationCrop improvementLinkageRecombinant haplotypeResistanceR-geneSingle nucleotide polymorphismSoybean (Glycine max (L.) Merrill)

Identifiers

PMID40426039
PMCPMC12117769

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