Evidence map›Paper›PMID 40425698›Full record

ArticleScientific reports2025

Serum expression signature of TUG1, MALAT1, miR-483, and miR-141 and their targets TGF-β1 and STAT3 in severe male factor infertility.

Mahmoud A Senousy, Olfat G Shaker, Ahmed Gamal, Nesreen M Aboraia, Ghada Ayeldeen

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PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Elevated LncRNAFASEB bioAdvances · 2026
    Article
  2. Observational
  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mahmoud A SenousyBiochemistry Department, Faculty of Pharmacy, Cairo University, Kasr Al Ainy st., Cairo, 11562, Egypt. mohmoud.ali@pharma.cu.edu.eg.
Olfat G ShakerMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Ahmed GamalAndrology, Sexology, and STIs Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Nesreen M AboraiaDermatology and STDs Department, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Ghada AyeldeenMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Long non-coding RNAs and microRNAs have evolved as biomarkers and specific therapeutic targets for male infertility. We investigated the seminal plasma and serum expression profiles of TUG1, MALAT1, miR-483, and miR-141 and their targets TGF-β1 and STAT3 in severe male factor infertility. The potential of their serum levels as minimally invasive, clinically accessible, and more feasible biomarkers and their clinical correlations were explored. Thirty non-obstructive azoospermia (NOA) patients, 30 severe oligozoospermia (SO) patients, and 30 healthy fertile men (controls) were enrolled. Compared to controls, seminal plasma and serum TUG1 and miR-141 were downregulated; meanwhile, miR-483 and STAT3 were upregulated in both NOA and SO patients, while MALAT1 was upregulated in NOA but downregulated in SO patients. Seminal plasma TGF-β1 was higher in NOA patients than in controls and SO patients, while its serum levels were not statistically different between the studied groups. NOA patients showcased higher serum STAT3 mRNA expression than SO patients. Seminal plasma and serum levels of most of the tested markers were correlated among NOA and SO patients. Multivariate logistic analysis unraveled the association of serum TUG1 and miR-141 with NOA risk and serum TUG1 and miR-483 with SO risk. A serum-based panel of TUG1/miR-141 and TUG1/miR-483 discriminated NOA (AUC = 0.93) and SO (AUC = 0.972) from controls, respectively. Serum miR-141 was correlated with the hormonal profile in NOA patients. In SO patients, serum TUG1 was correlated with total testosterone levels and abnormal sperm motility, while serum MALAT1 was inversely correlated with the testicular volume. Conclusively, this study introduces a novel serum-based prediction panel of TUG1/miR-141 and TUG1/miR-483 to help enhance the accuracy of NOA and SO diagnosis. Serum MALAT1 and STAT3 could be useful in stratifying NOA and SO. These findings warrant more future investigations for their possible implication in male infertility diagnostics.

Indexed as

Infertility, MaleMicroRNAsRNA, Long NoncodingSTAT3 Transcription FactorTransforming Growth Factor beta1AdultAzoospermiaBiomarkersCase-Control StudiesGene Expression RegulationHumansMaleOligospermiaSemenBiomarkersMALAT1 long non-coding RNA, humanMicroRNAsMIRN141 microRNA, humanRNA, Long NoncodingSTAT3 protein, humanSTAT3 Transcription FactorTGFB1 protein, humanTransforming Growth Factor beta1TUG1 long noncoding RNA, humanAzoospermiaInfertilityLncRNAMicroRNAOligozoospermia

Identifiers

PMID40425698
PMCPMC12116780

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.