Evidence map›Paper›PMID 40425660›Full record

ArticleScientific reports2025

An optimized method for directed differentiation of hypothalamic neural stem cells in a 3D culture system.

Jiao Luo, Qiaoyan Tang, Tanjing Lin, Jiabang Liu, Zhiheng Wu, Xintao Zhang, Xiaohua Zhang, Junhai Jiang, Yulong Wang

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PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Jiao Luo *Department of Rehabilitation Medicine, Dapeng New District Nan'ao People's Hospital, Rehabilitation Branch of the First Affiliated Hospital of Shenzhen University, Shenzhen, China.
Qiaoyan Tang *Department of Rehabilitation Medicine, Dapeng New District Nan'ao People's Hospital, Rehabilitation Branch of the First Affiliated Hospital of Shenzhen University, Shenzhen, China.
Tanjing LinSchool of Pharmacy, Guangdong Medical University, Guangdong, China.
Jiabang LiuShenzhen Institute of Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China.
Zhiheng WuDepartment of Rehabilitation Medicine, Dapeng New District Nan'ao People's Hospital, Rehabilitation Branch of the First Affiliated Hospital of Shenzhen University, Shenzhen, China.
Xintao ZhangDepartment of Urology, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, 518035, China.
Xiaohua ZhangDepartment of Rehabilitation Medicine, Dapeng New District Nan'ao People's Hospital, Rehabilitation Branch of the First Affiliated Hospital of Shenzhen University, Shenzhen, China. 21386658@qq.com.
Junhai JiangDepartment of Rehabilitation Medicine, Dapeng New District Nan'ao People's Hospital, Rehabilitation Branch of the First Affiliated Hospital of Shenzhen University, Shenzhen, China. 22248748@qq.com.
Yulong WangDepartment of Rehabilitation, Shenzhen Second People's Hospital, The First Affiliated Hospital, Shenzhen University School of Medicine, Shenzhen, China. ylwang668@163.com.

Funding

Natural Science Foundation of Guangdong Province 2020A1515011203Natural Science Funding of China 81901470Postdoctoral Science Foundation of China 2019M663100Sanming Project of Medicine in Shenzhen SZSM202111010Science, Technology and Innovation Commission of Shenzhen JCYJ20210324135804012Science, Technology and Innovation Commission of Shenzhen JCYJ20220530150407015Science, Technology and Innovation Commission of Shenzhen JCYJ20220530165211025
6 · The paper itself

Abstract

Hypothalamic neurogenesis is a complex process that plays a crucial role in neuroendocrine homeostasis, making in vivo studies of the hypothalamus particularly challenging. In this study, we present an optimized protocol for isolating and culturing hypothalamic neural stem cells (htNSCs) from neonatal (P1) mice, followed by their directed differentiation in a three-dimensional (3D) Matrigel environment. We successfully established a primary culture system that supports the stability, growth, and distinct characteristics of htNSCs. Notably, we demonstrate that htNSCs can differentiate into GnRH-like neurons within the Matrigel-based 3D culture system. These differentiated neurons exhibit typical neuronal morphology and functional characteristics. Our findings highlight the potential of neonatal htNSCs as an invaluable model for studying hypothalamic function and neurogenesis. Furthermore, this method provides a novel platform for basic research and may serve as important implications for further studying the pathological mechanism of neuroendocrine disorders in hypothalamus.

Indexed as

Cell Culture TechniquesCell Culture Techniques, Three DimensionalCell DifferentiationHypothalamusNeural Stem CellsAnimalsAnimals, NewbornCells, CulturedCollagenDrug CombinationsGonadotropin-Releasing HormoneLamininMiceNeurogenesisNeuronsProteoglycansCollagenDrug CombinationsGonadotropin-Releasing HormoneLamininmatrigelProteoglycansGnRH neuronHypothalamic neural stem cells (htNSCs)Neonatal miceNeuronal differentiationThree-dimensional culture

Identifiers

PMID40425660
PMCPMC12117107

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.