Evidence map›Paper›PMID 40423557›Full record

ArticleThe Journal of infectious diseases2025

Improving Molecular Epidemiological Surveillance of Strongyloidiasis Upon Differentiation of Strongyloides fuelleborni fuelleborni From Strongyloides stercoralis.

Lucas J Cunningham, William D Nevin, Jaco J Verweij, Dora Buonfrate, Salvatore Scarso, Virak Khieu, Angus M O'Ferrall, Sarah Rollason, J Russell Stothard

Abstract read
In one paragraph

Article in The Journal of infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Notes on the threadwormInternational journal for parasitology. Parasites and wildlife · 2025
    Article
  4. Article
  5. Review
  6. Genetic diversity withinParasitology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lucas J CunninghamDepartment of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, Merseyside, United Kingdom.ORCID 0000-0002-3017-4074
William D NevinDepartment of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, Merseyside, United Kingdom.ORCID 0009-0009-2747-8198
Jaco J VerweijMicrovida, Laboratory of Medical Microbiology and Immunology, Elisabeth-Tweesteden Hospital, Tilburg, The Netherlands.ORCID 0000-0001-8553-931X
Dora BuonfrateDepartment of Infectious, Tropical Diseases and Microbiology, Istituto di Ricovero e Cura a Carattere Scientifico Sacro Cuore Don Calabria Hospital, Verona, Italy.ORCID 0000-0003-0108-6822
Salvatore ScarsoDepartment of Infectious, Tropical Diseases and Microbiology, Istituto di Ricovero e Cura a Carattere Scientifico Sacro Cuore Don Calabria Hospital, Verona, Italy.ORCID 0000-0003-2956-8997
Virak KhieuMinistry of Health, National Centre for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.ORCID 0000-0002-1725-3843
Angus M O'FerrallDepartment of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, Merseyside, United Kingdom.ORCID 0000-0003-1077-968X
Sarah RollasonDepartment of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, Merseyside, United Kingdom.ORCID 0000-0003-4330-7099
J Russell StothardDepartment of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, Merseyside, United Kingdom.ORCID 0000-0002-9370-3420

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Molecular epidemiological surveillance for zoonotic strongyloidiasis is confounded by a genus-specific TaqMan probe assay that conflates Strongyloides fuelleborni fuelleborni with Strongyloides stercoralis. To improve surveillance, we developed and validated a novel duplex species-specific TaqMan probe assay, screening a representative collection of available clinical samples. Our assay was highly discriminatory, evidencing no cross-reactivity, and had a lowest DNA detection limit of 1 pg/µL. However, as the genus-specific DNA assay has greater detection ability, we propose a 2-step protocol where samples are first screened with this assay then, if positive, screened with our species-specific assay, discriminating (co)infections between each threadworm species.

Indexed as

StrongyloidesStrongyloides stercoralisStrongyloidiasisAnimalsDNA, HelminthEpidemiological MonitoringHumansMolecular EpidemiologySensitivity and SpecificityDNA, Helminthdiagnosticsreal-time PCRTaqMan assaythreadwormszoonosis

Identifiers

PMID40423557
PMCPMC12308652

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.