Evidence map›Paper›PMID 40422788›Full record

ArticleMarine drugs2025

Improvement of Catalytic Activity and Thermostability of Alginate Lyase VxAly7B-CM via Rational Computational Design Strategies.

Xin Ma, Ke Zhu, Kaiyang Wang, Wenhui Liao, Xiaohan Yang, Wengong Yu, Weishan Wang, Feng Han

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Marine drugs, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xin MaSchool of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Ke ZhuSchool of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Kaiyang WangSchool of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Wenhui LiaoSchool of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Xiaohan YangState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Wengong YuSchool of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Weishan WangSchool of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.ORCID 0000-0001-7827-2696
Feng HanSchool of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.ORCID 0000-0001-5706-7028

Funding

National Key R&D Program of China 2024YFC2816005Qingdao Marine Science and Technology Center 2022QNLM030003-1Science & Technology Development Project of Qingdao 22-3-6-gjxm-5-gx, 21-1-6-gjxm-27-gx and 20-11-6-64-gxShandong Province Key R&D Program 2023CXGC010414Shandong Province Technology Innovation Guidance Program 2018YFC0311105
6 · The paper itself

Abstract

Alginate lyase degrades alginate through the β-elimination mechanism to produce alginate oligosaccharides (AOS) with notable biochemical properties and diverse biological activities. However, its poor thermostability limits large-scale industrial production. In this study, we employed a rational computational design strategy combining computer-aided evolutionary coupling analysis and ΔΔG

Indexed as

Polysaccharide-LyasesAlginatesCatalysisEnzyme StabilityMolecular Dynamics SimulationTemperatureAlginatespoly(beta-D-mannuronate) lyasePolysaccharide-Lyasesalginate lyasealginate oligosaccharidesmolecular dynamics simulationrational designthermostability

Identifiers

PMID40422788
PMCPMC12112969

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.