Evidence map›Paper›PMID 40422061›Full record

ArticleBiosensors2025

Ultrasensitive and Real-Time Detection of Kanamycin Residues in Milk Using an Aptasensor Based on Microfluidic Capacitive Strategy.

Weidong Zheng, Jun Chai, Jayne Wu, Jian Zhang, Haochen Qi

Abstract read
In one paragraph

Article in Biosensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Weidong ZhengCollege of Electrical and Electronic Engineering, Wenzhou University, Wenzhou 325035, China.
Jun ChaiCollege of Electrical and Electronic Engineering, Wenzhou University, Wenzhou 325035, China.
Jayne WuDepartment of Electrical Engineering and Computer Science, The University of Tennessee, Knoxville, TN 37996, USA.ORCID 0000-0001-5143-9425
Jian ZhangCollege of Electrical and Electronic Engineering, Wenzhou University, Wenzhou 325035, China.
Haochen QiCollege of Electrical and Electronic Engineering, Wenzhou University, Wenzhou 325035, China.

Funding

National Natural Science Foundation of China 62074047Open Project of Key Laboratory of Architectural Acoustic Environment of Anhui Higher Education Institutes AAE2022KF01Open Research Fund of State Key Laboratory of Digital Medical Engineering 2024-M04Wenzhou Basic Scientific Research Project G20240024
6 · The paper itself

Abstract

Kanamycin (KanR) is a widely used antibiotic in human and veterinary medicine, as well as in food production and livestock breeding. However, its environmental residue and bioaccumulation in the food chain pose a great threat to human health. A real-time and sensitive aptasensor is developed for KanR detection based on a gold interdigitated electrode (IDE). A microfluidic alternating current electrothermal (ACET) effect is employed for rapid directional manipulation and enrichment of KanR molecules. As an ultrasensitive indicator, solid-liquid capacitance is adopted to reflect the tiny change on the IDE surface caused by target adsorption. The overall detection takes only 60 s from sample to result, and a wide linear detection range of 0.1 fM~1 pM, an ultra-low detection limit of 16.56 aM, and a high selectivity of 7752:1 are simultaneously achieved, with 5 times of repeated use and the shelf life of 10 days. Furthermore, the aptasensor shows excellent practicability in milk samples, with the spiked recovery rate ranging from 86.90% to 116.17%. This aptasensor with the detecting strategy provides a rapid, convenient, and cost-effective solution for real-time monitoring of KanR.

Indexed as

Aptamers, NucleotideBiosensing TechniquesKanamycinMilkAnimalsAnti-Bacterial AgentsDrug ResiduesElectrodesGoldLimit of DetectionAnti-Bacterial AgentsAptamers, NucleotideGoldKanamycinaptasensorinterface capacitancekanamycinmicrofluidic enrichment

Identifiers

PMID40422061
PMCPMC12110345

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.