Evidence map›Paper›PMID 40422013›Full record

ArticleBiosensors2025

Fluorescence Lifetime Multiplexing with Fluorogen-Activating FAST Protein Variants and Red-Shifted Arylidene-Imidazolone Derivative as Fluorogen.

Aidar R Gilvanov, Ivan N Myasnyanko, Sergey A Goncharuk, Marina V Goncharuk, Vadim S Kublitski, Daria V Bodunova, Svetlana V Sidorenko, Eugene G Maksimov, Mikhail S Baranov, Yulia A Bogdanova

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Reversible, Chemically Gated FRET via Ligand-Activated Acceptors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Aidar R GilvanovInstitute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.ORCID 0000-0001-6174-7798
Ivan N MyasnyankoInstitute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Sergey A GoncharukInstitute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.ORCID 0000-0002-0263-6462
Marina V GoncharukInstitute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.ORCID 0000-0002-6984-7125
Vadim S KublitskiInstitute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.ORCID 0000-0001-7142-2794
Daria V BodunovaDepartment of Biology, Lomonosov Moscow State University, Leninskye Gory, Build. 12, Moscow 119234, Russia.ORCID 0009-0006-4906-1930
Svetlana V SidorenkoDepartment of Biology, Lomonosov Moscow State University, Leninskye Gory, Build. 12, Moscow 119234, Russia.
Eugene G MaksimovDepartment of Biology, Lomonosov Moscow State University, Leninskye Gory, Build. 12, Moscow 119234, Russia.ORCID 0000-0001-6767-1501
Mikhail S BaranovInstitute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.ORCID 0000-0002-9339-7603
Yulia A BogdanovaInstitute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.ORCID 0000-0003-1745-4204

Funding

Russian Science Foundation 23-14-00042
6 · The paper itself

Abstract

Fluorescence-lifetime imaging microscopy (FLIM) is a powerful technique for highly multiplexed imaging in live cells. In this work, we present a genetically encoded FLIM multiplexing platform based on a combination of fluorogen-activating protein FAST and red-shifted fluorogen

Indexed as

Biosensing TechniquesFluorescent DyesImidazolesFluorescenceHumansMicroscopy, FluorescenceFluorescent DyesImidazolesimidazolonearylidene–imidazoloneFLIMfluorogen-activating protein

Identifiers

PMID40422013
PMCPMC12109237

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.