Evidence map›Paper›PMID 40421117›Full record

ArticleFrontiers in bioengineering and biotechnology2025

Deletion of succinic semialdehyde dehydrogenase sad and chromosomal expression of phosphoenolpyruvate carboxylase as metabolic requirements for improved production of 2,4-dihydroxybutyric acid via malyl-P pathway using

T A Stefanie Nguyen, Ceren Alkim, Nadine Ihle, Thomas Walther, Cláudio J R Frazão

Abstract read
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In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

T A Stefanie NguyenChair of Bioprocess Engineering, Institute of Natural Materials Technology, TU Dresden, Dresden, Germany.
Ceren AlkimToulouse Biotechnology Institute, UMR INSA-CNRS5504, UMR INSA-INRAE 792, Toulouse, France.
Nadine IhleChair of Bioprocess Engineering, Institute of Natural Materials Technology, TU Dresden, Dresden, Germany.
Thomas WaltherChair of Bioprocess Engineering, Institute of Natural Materials Technology, TU Dresden, Dresden, Germany.
Cláudio J R FrazãoChair of Bioprocess Engineering, Institute of Natural Materials Technology, TU Dresden, Dresden, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The fermentative production of the functional precursor 2,4-dihydroxybutyrate (DHB) enables sustainable synthesis of the methionine analogue hydroxy-4-(methylthio) butyrate, which is currently still produced from fossil fuels. In this work, we aimed to optimize the aerobic production of DHB from glucose through the synthetic malyl phosphate (MalP) pathway, which comprises the conversion of the natural TCA cycle intermediate malate into MalP and the subsequent reactions to yield malate semialdehyde (MalSA) and finally DHB. We first implemented the synthetic pathway in an engineered

Indexed as

2,4-dihydroxybutyric acidEscherichia colimalyl phosphate pathwayphosphoenolpyruvate carboxylasestrain engineeringsuccinic semialdehyde dehydrogenasesynthetic metabolic pathway

Identifiers

PMID40421117
PMCPMC12104201

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.