ArticleInfectious diseases of poverty2025
Combination of Cpb-Hsp70 typing methods reveals genetic divergence between Leishmania infantum strains causing human tegumentary leishmaniasis in northern Italy and central Spain: a retrospective study.
Article in Infectious diseases of poverty, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Evaluation of Multi-Target Genotyping (ITS-Pathogens (Basel, Switzerland) · 2026Article
- Characterization ofVeterinary sciences · 2025Article
- Rare detection of dermalParasite epidemiology and control · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
backgroundTegumentary leishmaniasis (TL) caused by Leishmania infantum is an overlooked yet re-emerging disease endemic in Mediterranean Europe. Currently, no standardized molecular surveillance of circulating Leishmania strains is performed in European endemic areas, despite the potential public health implications of parasite biodiversity. This study aims to characterize parasite population haplogroups causing TL in two active endemic areas in southern Europe, i.e. Bologna (northern Italy) and Fuenlabrada (central Spain).
methodsIn this retrospective study, we typed 87 L. infantum samples from TL cases in the areas of Bologna and Fuenlabrada; these areas hosted the main European foci of human TL occurring in the last 15 years. Two Leishmania genomic typing targets were used: the heat shock protein 70 (Hsp70) and the cysteine peptidase b (Cpb). Simpson's index was used to calculate the discriminatory power of the used typing methods.
resultsTyping results depicted the presence of a heterogeneous parasite population circulating in Bologna with two main haplogroups, i.e. Hsp70(A)_Cpb(F) (n = 7, 30.4%) and Hsp70(G)_Cpb(E/F) (n = 7, 30.4%), differing from the reference L. infantum strain JPCM5 haplogroup and partially overlapping with L. donovani lineages. Among the samples from Fuenlabrada, n = 19 samples were typed by both targets, revealing a homogeneous population expressing Hsp70(A) and Cpb(E), matching the JPCM5 reference strain haplogroup. Overall, the Cpb typing method exhibited higher discrimination power as compared to the Hsp70 method (Simpson's index of diversity, P-value < 0.05).
conclusionsOur findings show differences among L. infantum populations causing TL in two southern European epidemiological foci of human leishmaniasis and support the recent discovery of L. infantum/L.donovani hybrid strains circulating in northern Italy. These results underscore the critical need to identify the circulating Leishmania strains in endemic areas and assess their potential public health implications in active foci.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.