Evidence map›Paper›PMID 40420057›Full record

ArticleBMC genomics2025

A Mg

Ao Li, Bingrui Sun, Ying Zhang, Ping Yu, Jicheng Qu, Hongkuan Deng, Qiuxiang Pang, Fengtang Yang

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Ao LiSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255049, PR China. aoli@sdut.edu.cn.
Bingrui SunSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255049, PR China.
Ying ZhangSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255049, PR China.
Ping YuSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255049, PR China.
Jicheng QuSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255049, PR China.
Hongkuan DengSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255049, PR China.
Qiuxiang PangSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255049, PR China. pangqiuxiang@sdut.edu.cn.
Fengtang YangSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255049, PR China. fengtangyang@163.com.

Funding

Natural Science Foundation of China 31701845Natural Science Foundation of China 32370689Natural Science Foundation of China 32470518Natural Science Foundation of Shandong Province, China ZR2022MC022Natural Science Foundation of Shandong Province, China ZR2024MC154
6 · The paper itself

Abstract

backgroundThe isolation of intact, high-molecular-weight genomic DNA (HMW gDNA) is essential for achieving complete genome assemblies. However, extracting HMW gDNA from a single individual of Dugesia japonica remains a technical challenge using the standard protocol, probably due to the presence of abundant polysaccharides and nucleases.

resultsIn this study, we have developed a more robust protocol for preparing HMM gDNA, with high yields and quality, from a single D. japonica. The key step in our protocol involves the use of a Mg

conclusionsThis protocol offers a simple and high-yield solution for isolating HMW gDNA from D. japonica. It also provides an alternative for organisms whose gDNA consistently exhibits unexplained degradation using established protocols.

Indexed as

DNADNA, HelminthGenomicsMagnesiumPlanariansAnimalsMolecular WeightDNADNA, HelminthMagnesiumDeoxyribonuclease IIHigh-molecular-weight genomic DNA extractionHigh yieldMg2+Planarian

Identifiers

PMID40420057
PMCPMC12107755

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.