Evidence map›Paper›PMID 40419962›Full record

ArticleBMC genomics2025

Identification of single nucleotide polymorphisms (SNPs) associated with heat stress and milk production traits in Chinese holstein cows.

Jingyi Xu, Lirong Hu, Jingyang Ning, Fan Zhang, Qing Xu, Yachun Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jingyi XuCountry National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Lirong HuCountry National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Jingyang NingCountry National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Fan ZhangCountry National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Qing XuCollege of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, 100044, China.
Yachun WangCountry National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China. wangyachun@cau.edu.cn.

Funding

Ministry of Finance and Ministry of Agriculture and Rural Affairs: National modern agricultural industry technology system CARS-36Program for Changjiang Scholar and Innovation Research Team in University IRT_15R62
6 · The paper itself

Abstract

backgroundHeat stress (HS) poses a significant challenge to the dairy industry, affecting both the health and productivity of dairy cows. Identifying candidate genes and single nucleotide polymorphisms (SNPs) associated with HS is critical to improving heat tolerance of dairy cows. In our previous work, the eukaryotic translation initiation factor 4E (EIF4E), heat shock protein family A member 4 (HSPA4), and inositol 1,4,5-trisphosphate receptor type 2 (ITPR2) genes were found to play critical roles in the HS response of dairy cows.

resultsIn this study, we further validated the gene expression patterns and genetic effects of the three candidate genes on HS response and milk production in Chinese Holstein cows. A total of 21 SNPs were identified by sequencing the exon and 2000 bp flanking region of the EIF4E (4 SNPs), HSPA4 (8 SNPs), and ITPR2 (9 SNPs) in pooled DNA samples from 70 Holstein bulls. Among these, two SNPs (g.44653172A > G and g.44660065C > T) were located in the coding exon and the 3' untranslated region of the HSPA4 gene, respectively. Association analyses were conducted between identified SNPs and three HS traits and six milk production traits in a population of 1,160 Chinese Holstein cows. The SNP-based association analysis identified significant associations between ten SNPs and HS traits (P < 0.05), as well as eight SNPs and milk production traits (P < 0.05). In the HSPA4 gene, five SNPs (g.44618036G > A, g.44624256 A > C, g.44624428T > C, g.44653172 A > G, and g.44660065 C > T) were significantly associated with rectal temperature (RT; P < 0.05). Notably, no SNPs were associated with milk production traits. Haplotype blocks containing g.44,653,172 A > G and g.44,660,065 C > T also showed significant associations with RT (P < 0.05). Further analysis suggests that g.44,660,065 C > T affects the stability of the mRNA secondary structure, microRNA and transcription factor binding, thereby potentially influencing the gene expression of HSPA4.

conclusionIn conclusion, we demonstrated that these three genes had significant genetic effects on HS and milk production traits. And g.44,660,065 C > T in the HSPA4 gene could be used as functional molecular markers for the genetic selection of dairy cows for improving heat tolerance without compromising their high milk performance.

Indexed as

Heat-Shock ResponseLactationMilkPolymorphism, Single NucleotideQuantitative Trait, HeritableAnimalsCattleEukaryotic Initiation Factor-4EFemaleGenetic Association StudiesHaplotypesInositol 1,4,5-Trisphosphate ReceptorsMaleEukaryotic Initiation Factor-4EInositol 1,4,5-Trisphosphate ReceptorsAssociation analysisDairy cowsHeat stressHSPA4 geneMilk production traits

Identifiers

PMID40419962
PMCPMC12107732

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.