Evidence map›Paper›PMID 40419845›Full record

ArticleRice (New York, N.Y.)2025

OsSDG715, a Histone H3K9 Methyltransferase, Integrates Auxin and Cytokinin Signaling to Regulate Callus Formation in Rice.

Wenjing Song, Hairong Cai, Yuanyuan Guo, Shiyi Chen, Yingyun Yao, Jiafeng Wang, Tao Guo, Jian Zhang, Chun Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Rice (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wenjing SongNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, 510642, China.
Hairong CaiNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, 510642, China.
Yuanyuan GuoNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, 510642, China.
Shiyi ChenNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, 510642, China.
Yingyun YaoNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, 510642, China.
Jiafeng WangNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, 510642, China.
Tao GuoNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, 510642, China.
Jian ZhangInstitute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China. jianzhang@scsaas.cn.
Chun ChenNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, 510642, China. chchun@scau.edu.cn.

Funding

Construction of a Rice Cosmic Particle-Oriented Mutation Technology System and Creation of New Germplasm 2022YFD1200703Integrated Demonstration of High-Quality, High-Yield, and High-Resistance Space Rice Varieties 2023B0202010003
6 · The paper itself

Abstract

Efficient callus induction is essential for the genetic transformation of rice (Oryza sativa), yet its regulatory mechanisms remain elusive. Previously, through a genome-wide association study (GWAS), we identified a significant associated locus on chromosome 8. In this study, we characterized this locus and demonstrated that OsSDG715, encoding a histone H3K9 methyltransferase, is the causal gene that positively regulates callus formation in rice. Results revealed that OsSDG715 is highly expressed during callus induction and exhibits natural variations associated with callus induction rate (CIR). Knockout of OsSDG715 via CRISPR/Cas9 led to a significant decrease in CIR and impaired callus morphology, indicating its positive regulation of callus formation. RNA-seq analyses revealed that 326 and 705 differentially expressed genes (DEGs) were upregulated and downregulated in sdg715 mutants, including auxin-responsive genes (OsIAA14, OsYUCCA6), cytokinin-related genes (OsCKX4, ARR10), and stress-responsive factors. Further analysis showed reduced endogenous indole-3-acetic acid (IAA) levels and increased zeatin levels in sdg715 mutants. These findings advance our understanding of the molecular mechanisms underlying rice callus formation, and offering valuable insights for optimizing tissue culture in molecular breeding.

Indexed as

AuxinCallus formationCytokininOsSDG715Rice

Identifiers

PMID40419845
PMCPMC12106170

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.