Evidence map›Paper›PMID 40419764›Full record

ArticleBiomolecular NMR assignments2025

Backbone and sidechain

Benjamin D Harding, Ashley Hiett, Marco Tonelli, Songlin Wang, Chad M Rienstra, Katherine A Henzler-Wildman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecular NMR assignments, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Protonated Structure of EmrE Reveals C-terminal Tail Gating Mechanism.bioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Benjamin D Harding *Biophysics Graduate Program, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Ashley Hiett *Biophysics Graduate Program, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Marco TonelliDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Songlin WangDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Chad M RienstraBiophysics Graduate Program, University of Wisconsin-Madison, Madison, WI, 53706, USA. crienstra@wisc.edu.
Katherine A Henzler-WildmanDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA. henzlerwildm@wisc.edu.

Funding

NMR User Program at NMRFAMR24GM141526 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Katherine Anne Henzler-Wildman · 2021 to 2026
$6.4M
Molecular Mechanisms of Channels and TransportersR35GM141748 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Katherine Anne Henzler-Wildman · 2021 to 2026
$2.7M
Acquisition of Bruker 900 MHz NEO console and probesS10OD034243 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI HENZLER-WILDMAN, KATHERINE ANNE · 2023 to 2023
$1.5M
Predoctoral Training in Molecular BiophysicsT32GM130550 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SENES, ALESSANDRO · 2019 to 2023
$1.5M
NIGMS NIH HHS R24 GM141526NIGMS NIH HHS R35 GM141748NIGMS NIH HHS T32 GM130550NIH HHS R24GM141526NIH HHS S10 OD034243NIH HHS T32GM130550NSF 1946970
6 · The paper itself

Abstract

EmrE is a bacterial membrane-embedded multidrug transporter that functions as an asymmetric homodimer. EmrE is implicated in antibiotic resistance but is now known to confer either resistance or susceptibility depending on the identity of the small molecule substrate. Here, we report both solution- and solid-state NMR assignments of S64V-EmrE at pH 5.8, below the pKa of critical residues E14 and H110. This includes

Indexed as

AntiportersEscherichia coli ProteinsNuclear Magnetic Resonance, BiomolecularCarbon IsotopesNitrogen IsotopesSolutionsAntiportersCarbon IsotopesEmrE protein, E coliEscherichia coli ProteinsNitrogen-15Nitrogen IsotopesSolutionsAntibiotic resistanceEmrEMagic-angle spinningMembrane proteinNMR spectroscopySolid-state NMR

Identifiers

PMID40419764
PMCPMC12513983

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.