ArticleLab animal2025
Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study.
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Article in Lab animal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11citing papers in PubMed
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Who cites it
11 citing papers in PubMed.
- zAcademy: an open-source programmable conditioning arena reveals sex- and genotype-dependent avoidance learning in adult zebrafish.bioRxiv : the preprint server for biology · 2026Article
- The sensitivity of adult zebrafish to clinically relevant neuroactive (sedative) effects of extracts of selected traditional Chinese medicine herbs.Psychopharmacology · 2026Article
- Neurobehavioral Effects of Two Distinct Anti-Inflammatory Drugs in a Two-Week Chronic Stress Model in Zebrafish.Brain sciences · 2026Article
- BDNF Deficiency Preserves Shoal Structure but Selectively Modulates Horizontal Exploration in an Adult BDNFInternational journal of molecular sciences · 2026Article
- Mechanistic study on the alleviating effects of cinnamaldehyde on aluminum chloride-induced cognitive impairment in zebrafish through modulating the TLR4/NF-κB signaling pathway.Metabolic brain disease · 2026Article
- Zebrafish: a key model for unraveling endocrine-disrupting effects on thyroid development and function.Frontiers in pharmacology · 2026Review
- Stress Coping Style Alters Functional Brain Network Activity to Acute Stressor.bioRxiv : the preprint server for biology · 2025Article
- Distinct Swimming Behavioral Phenotypes Following Serotonin and Dopamine Transporter Modulation in the Adult Zebrafish Novel Tank Diving Test (NTT).Pharmaceuticals (Basel, Switzerland) · 2025Article
- Zebrafish as a Model Organism for Post-Traumatic Stress Disorder: Insights into Stress Mechanisms and Behavioral Assays.Biology · 2025Review
- Personality Traits of the Territorial Crustacean Chinese Mitten Crab (Animals : an open access journal from MDPI · 2025Article
- Refinement of the novel tank diving test: toward standardized and robust analysis of anxiety-like behavior in zebrafish.Frontiers in behavioral neuroscience · 2025Article
Corrections and comments
- Update of
Authors and funding
52 authors.
Courtney Hillman *Surrey Sleep Research Centre, Department of Clinical and Experimental Medicine, School of Biosciences, University of Surrey, Surrey, UK.ORCID 0000-0003-4787-7400
Barbara D Fontana *Laboratory of Experimental Neuropsychobiology, Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.ORCID 0000-0003-2832-400X
Tamara G AmstislavskayaLaboratory of Experimental Models of Neuropsychiatric Disorders, Scientific Research Institute of Neurosciences and Medicine, Novosibirsk State University, Novosibirsk, Russia.
Maria A GorbunovaUral Federal University, Yekaterinburg, Russia.
Stefani AltenhofenLaboratório de Neuroquímica e Psicofarmacologia, Escola de Ciências da Saúde e da Vida, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.ORCID 0000-0001-7229-6494
Karissa BarthelsonAlzheimer's Disease Genetics Laboratory, School of Biological Sciences, Faculty of Sciences, Engineering and Technology, The University of Adelaide, Adelaide, South Australia, Australia.
Leonardo M BastosLaboratório de Psicofarmacologia e Comportamento, Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.ORCID 0000-0001-8538-6405
João V BorbaLaboratory of Experimental Neuropsychobiology, Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
Carla D BonanLaboratório de Neuroquímica e Psicofarmacologia, Escola de Ciências da Saúde e da Vida, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.ORCID 0000-0001-9715-6244
Caroline H BrennanSchool of Biological and Behavioural Sciences, Queen Mary University of London, London, UK.ORCID 0000-0002-4169-4083
Amaury Farías-CeaMolecular Pharmacology and Medicinal Chemistry Lab, Facultad de Ingeniería y Ciencias, Universidad de la Frontera, Temuco, Chile.ORCID 0000-0001-5468-4936
Austin CooperSurrey Sleep Research Centre, Department of Clinical and Experimental Medicine, School of Biosciences, University of Surrey, Surrey, UK.
Jamie CorcoranDepartment of Psychology, University of Nebraska at Omaha, Omaha, NE, USA.
Eduardo R DondossolaTranslational Psychiatry Laboratory, Graduate Program in Health Sciences, University of Southern Santa Catarina, Criciúma, Brazil.
Luis M Martinez-DuranMolecular Pharmacology and Medicinal Chemistry Lab, Facultad de Ingeniería y Ciencias, Universidad de la Frontera, Temuco, Chile.
Matheus Gallas-LopesLaboratório de Psicofarmacologia e Comportamento, Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.ORCID 0000-0001-5375-2335
David S GalstyanSt. Petersburg State University, St. Petersburg, Russia.ORCID 0000-0001-6220-3485
Ella O GarciaDepartment of Psychology, Indiana University Northwest, Gary, IN, USA.
Ewan GerkenAlzheimer's Disease Genetics Laboratory, School of Biological Sciences, Faculty of Sciences, Engineering and Technology, The University of Adelaide, Adelaide, South Australia, Australia.ORCID 0000-0002-5654-8556
Robert HindgesCentre for Developmental Neurobiology and MRC Centre for Neurodevelopmental Disorders, King's College London, London, UK.ORCID 0000-0003-0095-7913
Justin W KenneyDepartment of Biological Sciences, Wayne State University, Detroit, MI, USA.ORCID 0000-0001-8790-5184
Maxim A KleshchevLaboratory of Experimental Models of Neuropsychiatric Disorders, Scientific Research Institute of Neurosciences and Medicine, Novosibirsk State University, Novosibirsk, Russia.
Tatiana O KolesnikovaNeurobiology Program, Sirius University of Science and Technology, Sochi, Russia.
Adele LeggieriSchool of Biological and Behavioural Sciences, Queen Mary University of London, London, UK.ORCID 0000-0003-0449-9665
Sergey L KhatskoUral Federal University, Yekaterinburg, Russia.ORCID 0000-0001-5921-6680
Michael LardelliAlzheimer's Disease Genetics Laboratory, School of Biological Sciences, Faculty of Sciences, Engineering and Technology, The University of Adelaide, Adelaide, South Australia, Australia.
Guilherme LodettiTranslational Psychiatry Laboratory, Graduate Program in Health Sciences, University of Southern Santa Catarina, Criciúma, Brazil.
Giulia LombardelliDepartment of Mechanical and Aerospace Engineering, Department of Biomedical Engineering, Center for Urban Science and Progress, New York University, Tandon School of Engineering, New York, NY, USA.
Ana C LuchiariFishLab, Department of Physiology and Behavior, Federal University of Rio Grande do Norte, Natal, Brazil.
Stefani M PortelaLaboratório de Psicofarmacologia e Comportamento, Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Violeta MedanInstituto de Fisiología, Biología Molecular y Neurociencias, Consejo Nacional de Investigaciones Científicas y Tecnológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.ORCID 0000-0002-3612-0659
Lirane M MoutinhoInstituto de Fisiología, Biología Molecular y Neurociencias, Consejo Nacional de Investigaciones Científicas y Tecnológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Evgeny V NekhoroshevLaboratory of Experimental Models of Neuropsychiatric Disorders, Scientific Research Institute of Neurosciences and Medicine, Novosibirsk State University, Novosibirsk, Russia.
Barbara D PetersenLaboratório de Neuroquímica e Psicofarmacologia, Escola de Ciências da Saúde e da Vida, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
Maureen L Petrunich-RutherfordDepartment of Psychology, Indiana University Northwest, Gary, IN, USA.
Angelo PiatoLaboratório de Psicofarmacologia e Comportamento, Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.ORCID 0000-0001-5109-7306
Maurizio PorfiriDepartment of Mechanical and Aerospace Engineering, Department of Biomedical Engineering, Center for Urban Science and Progress, New York University, Tandon School of Engineering, New York, NY, USA.ORCID 0000-0002-1480-3539
Emily ReadCentre for Developmental Neurobiology and MRC Centre for Neurodevelopmental Disorders, King's College London, London, UK.
Cássio M ResmimLaboratory of Experimental Neuropsychobiology, Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
Eduardo P RicoTranslational Psychiatry Laboratory, Graduate Program in Health Sciences, University of Southern Santa Catarina, Criciúma, Brazil.
Denis B RosembergLaboratory of Experimental Neuropsychobiology, Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.ORCID 0000-0003-2538-8761
Murilo S de AbreuGraduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, Brazil.ORCID 0000-0001-5562-0715
Catia A SalazarDepartment of Psychology, Indiana University Northwest, Gary, IN, USA.
Thailana Stahlhofer-BussLaboratório de Psicofarmacologia e Comportamento, Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.ORCID 0000-0003-2183-3493
Júlia R TeixeiraFishLab, Department of Physiology and Behavior, Federal University of Rio Grande do Norte, Natal, Brazil.
Ana M ValentimLaboratory Animal Science, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, i3S, Porto, Portugal.ORCID 0000-0003-1428-0691
Alexander V ZhdanovUral Federal University, Yekaterinburg, Russia.
Patricio Iturriaga-VásquezMolecular Pharmacology and Medicinal Chemistry Lab, Facultad de Ingeniería y Ciencias, Universidad de la Frontera, Temuco, Chile.
Xian WangSchool of Biological and Behavioural Sciences, Queen Mary University of London, London, UK.
Ryan Y WongDepartment of Psychology, University of Nebraska at Omaha, Omaha, NE, USA.ORCID 0000-0003-0236-672X
Allan V KalueffLaboratory of Experimental Neuropsychobiology, Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil. avkalueff@gmail.com.ORCID 0000-0002-7525-1950
Matthew O ParkerSurrey Sleep Research Centre, Department of Clinical and Experimental Medicine, School of Biosciences, University of Surrey, Surrey, UK. matthew.parker@surrey.ac.uk.ORCID 0000-0002-7172-5231
Funding
UNMC Structural Biology CoreP20GM103427 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI Heather Colleen Jensen-Smith · 2012 to 2026
$59.2MExploiting zebrafish genetics to identify genes affecting addiction-related phenotypes.U01DA044400 · NIDA · UNIV/LONDON-QUEEN MARY& WESTFIELD COLL · PI BRENNAN, CAROLINE HELEN, BUSCH-NENTWICH, ELISABETH MARIE · 2018 to 2022
$2.0MNeural and Molecular Basis of Individual Differences in Brain Function and BehaviorR35GM142566 · NIGMS · WAYNE STATE UNIVERSITY · PI KENNEY, JUSTIN WARD · 2021 to 2025
$1.9MNeural and Molecular Mechanisms in Stress CopingR15MH113074 · NIMH · UNIVERSITY OF NEBRASKA OMAHA · PI WONG, RYAN YING · 2017 to 2017
$346kMOD | Defence Science and Technology Laboratory (Dstl) n/aNational Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) NC/W00092X/1NIDA NIH HHS U01 DA044400NIGMS NIH HHS P20 GM103427NIGMS NIH HHS R35 GM142566NIMH NIH HHS R15 MH113074
Abstract
The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes. Global collaborative studies offer a unique solution to this problem. Here, we present a global collaboration using the zebrafish (Danio rerio) novel tank test, a popular behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show that female zebrafish exhibit more anxiety-like behavior than males, highlighting sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, also influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (for example, rotifers) mitigated anxiety-like behaviors. Environmental stressors, such as noise and transportation, significantly impacted behavior. We recommend standardizing testing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds and minimizing stressors to improve the reliability of zebrafish behavioral studies.
Indexed as
Animal HusbandryBehavior, AnimalHousing, AnimalZebrafishAnimal FeedAnimalsAnxietyFemaleMaleReproducibility of ResultsSex Factors
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.