ArticleAnatomical science international2025
Advanced fixation techniques for high-sensitivity molecular imaging: effectiveness of glyoxal fixation for immunostaining.
Article in Anatomical science international, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Improvement and development of immersion fixatives for enhanced tissue preservation in the whole eyeball specimens.The Journal of veterinary medical science · 2025Article
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3 authors.
Funding
Abstract
Chemical fixation is essential in immunohistochemistry for detecting molecular localization. The gold-standard fixative for immunohistochemistry is 4% paraformaldehyde (PFA); however, its properties-such as tissue shrinkage and protein cross-linking through methylene bridges-often restrict antibody access, posing challenges to achieving specific binding reactions. This is particularly true for receptors and ion channels condensed in the synaptic cleft, postsynaptic density, or trigger zone of action potentials. To overcome this problem, several laboratories have attempted antigen-exposure techniques. Recently, we demonstrated that fixation by glyoxal, a dialdehyde with two carbon atoms, enables specific detection of molecular groups that are difficult to be detected in PFA-fixed tissues. Here, we summarize the advantages and precautions in the use of glyoxal fixative.
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Registered trials
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