ArticleMethods in molecular biology (Clifton, N.J.)2025
DCFDA Assay for Oxidative Stress Measurement in Fluorometer.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Fungal Melanins as Potential Reactive Oxygen Species-Scavenging Neuroprotective Agents.Molecules (Basel, Switzerland) · 2026Article
- Nuclear remodeling and optimal migration emerge early, and constriction-passage progressively improves during hematopoietic stem cells to neutrophils differentiation.bioRxiv : the preprint server for biology · 2026Article
- Mechanistic Insights into Nrf2 Activation and Oxidative Stress Responses of Per- and Polyfluoroalkyl Substances (PFAS) in Human Keratinocytes.Biomolecules & therapeutics · 2026Article
- Holotomography-Based, Label-Free Quantification of Cellular Dry Mass as a Biophysical Indicator of Microglial Aβ Phagocytosis during Senescence.Research square · 2026Article
- Ring-fused chlorin-enhanced photodynamic therapy for effective cell death induction in endometrial cancer.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
Corrections and comments
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Authors and funding
1 author.
Funding
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Abstract
Cells manage oxidative stress from endogenous and exogenous stimuli. Excess oxidative stress causes significant cellular health issues, impacting key biological components such as DNA, membrane, and proteins. Reactive oxygen species (ROS) are a major contributor to oxidative stress, making the measurement of oxidative stress crucial for monitoring cellular health. DCFDA (2',7'-dichlorofluorescin diacetate) is a widely used probe for detecting oxidative stress in live cells. Once inside the cell, DCFDA is converted to DCFH, which is then oxidized to produce the fluorescent compound DCF. The green fluorescence emitted by DCF under blue light exposure serves as an indicator of oxidative stress. However, DCFDA is a broad oxidative probe and does not provide specificity for different types of ROS. This chapter introduces the use of carboxy-H
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