Radiation-Induced DNA Strand Break Detection by Gel Electrophoresis.

Dylan J Buglewicz, Takamitsu A Kato

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Immune and inflammatory radiogenomics of radiotherapy-induced normal-tissue toxicity: genetic susceptibility, mechanisms, risk prediction, and clinical translation.Frontiers in immunology · 2026
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Dylan J BuglewiczCenter for Computational Toxicology and Exposure, Office of Research and Development, US Environmental Protection Agency, Duluth, MN, USA. dylan.buglewicz@alumni.colostate.edu.

Takamitsu A KatoDepartment of Environmental & Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA double-strand breaks (DSBs), from localized DNA single-strand breaks (SSBs), are the most toxic DNA lesions induced by ionizing radiation and are the major actor responsible for radiation-induced cell death. Ionizing radiation-induced DSBs are not only influenced by the dosage of irradiation but are also by whether the radiation source has high- or low-linear energy transfer (LET), such as carbon-ion or X-ray beams, respectfully. The ability to induce DSBs by each source of irradiation can be observed at increasing dosages by agarose gel electrophoresis. In this chapter, we present a technique using agarose gel electrophoresis that provides both a simple and effective way to compare differences behind DNA damage capabilities of ionizing radiation sources of increasing LET and how it can also be a simple method to test certain chemicals for their ability to either increase (radiosensitization) or decrease (radioprotection) the DNA damage associated with each source of radiation in a controlled environment.

Indexed as

DNADNA Breaks, Double-StrandedDNA DamageElectrophoresis, Agar GelHumansLinear Energy TransferRadiation, IonizingDNAAgarose gel electrophoresisCarbon-ionDNA damageHigh-LETIonizing radiationLow-LET

Identifiers

PMID40418468

DOI10.3390/toxics11090773

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.