Imaging Translation in Early Embryo Development.

Pierre Bensidoun, Morgane Verbrugghe, Mounia Lagha

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Pierre BensidounInstitut de Génétique Moléculaire de Montpellier, CNRS UMR 5535, University of Montpellier, Montpellier, France.

Morgane VerbruggheInstitut de Génétique Moléculaire de Montpellier, CNRS UMR 5535, University of Montpellier, Montpellier, France.

Mounia LaghaInstitut de Génétique Moléculaire de Montpellier, CNRS UMR 5535, University of Montpellier, Montpellier, France. mounia.lagha@igmm.cnrs.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ultimate output of gene expression is to ensure that proteins are synthesized at the right levels, locations, and timings. Recently different imaging-based methods have been developed to visualize the translation of single mRNA molecules. These methods rely on signal amplification with the introduction of an array of a short peptide sequence (a tag such as SunTag), recognized by a genetically encodable single-chain antibody (a detector such as scFv). In this chapter, we discuss such methods to image and quantify translation dynamics in the early Drosophila embryo and provide examples based on a twist-32XSunTag reporter. We outline a step-by-step protocol to light-up translation in living embryos. We also detail a combinatorial strategy in fixed samples (smFISH-IF), allowing to distinguish single mRNA molecules engaged in translation.

Indexed as

Embryonic DevelopmentProtein BiosynthesisAnimalsDrosophilaDrosophila melanogasterEmbryo, NonmammalianGene Expression Regulation, DevelopmentalIn Situ Hybridization, FluorescenceRNA, MessengerSingle Molecule ImagingRNA, MessengerCell biologyDevelopmental biologyDrosophilaEmbryoGene expressionLive imagingsmFISHSunTag/scFvTranslation

Identifiers

PMID40418452

DOI10.1016/j.molcel.2021.01.029

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.