Evidence map›Paper›PMID 40418440›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Probing the Genomic Distribution of Histone Modifications in Egg Extract-Treated Sperm Nuclei.

Florian Berger, Jérôme Jullien

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Florian BergerNantes Université, CHU Nantes1, Ecole Centrale Nantes1, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ITUN2, Nantes, France.
Jérôme JullienNantes Université, CHU Nantes1, Ecole Centrale Nantes1, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ITUN2, Nantes, France. jerome.jullien@inserm.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Monitoring the fate of epigenetic cues delivered by sperm to the embryo can be challenging due to the paucity of available material. The use of Xenopus laevis egg extract to mimic the chromatin events associated with the first embryonic division provide an alternative approach to the use of embryos. In this chapter, we describe a protocol to carry out quantitative ChIP-seq analysis of modified histones fate following sperm exposure to maternal factor components to understand the relevance of male-derived histone modifications during embryogenesis.

Indexed as

Cell NucleusHistone CodeHistonesOvumSpermatozoaAnimalsChromatinChromatin Immunoprecipitation SequencingEmbryonic DevelopmentEpigenesis, GeneticFemaleMaleProtein Processing, Post-TranslationalXenopus laevisChromatinHistonesChIP-seqEgg extractModified histoneSpermXenopus

Identifiers

PMID40418440

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.