Development of one-step multiplex real-time PCR for the detection of CHV-1, CAdV-2, and CDV.

Yifan Li, Jingqi Wu, Chaonan Wang, Zheng Jia, Zaixing Yang, Wei Lin, Junwei Ge, Lili Zhao

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Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Yifan LiState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China.

Jingqi WuState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China.

Chaonan WangCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Zheng JiaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Zaixing YangCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Wei LinState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China.

Junwei GeCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Lili ZhaoState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Canine Infectious Respiratory Disease Complex (CIRDC) is a highly contagious disease that frequently affects canine populations and has emerged as a global epidemic. It has been reported that CIRDC can have a serious impact on related life. Therefore, the rapid detection and differentiation of common viruses that cause CIRDC are essential. It is generally believed that CIRDC is mainly caused by infection of three pathogens: canine herpesvirus-1 (CHV-1), canine adenovirus-2 (CAdV-2), and canine distemper virus (CDV). In this study, we developed and validated a TaqMan probe-based multiplex real-time PCR method to detect and identify these three viruses simultaneously. We designed specific primers and probes, and optimized the concentrations of each reactant in the system. The method was found to have good sensitivity, specificity and stability, and had a limit of detection of 10

Indexed as

canine adenovirus-2canine distemper viruscanine herpesvirus-1CIRDCmultiplex real-time PCR

Identifiers

PMID40417353

DOI10.3389/fvets.2025.1583769

PMCPMC12098444

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.