Evidence map›Paper›PMID 40416648›Full record

ArticleRegenerative biomaterials2025

Differentiation of human induced pluripotent stem cells into retinal pigment epithelium cells during culture on peptide-grafted hydrogels.

Jun Liu, Qian Liu, Minmei Guo, Chengyu Jiang, Jianyang Chen, Ting Wang, Tzu-Cheng Sung, Shih-Jie Chou, Shih-Hwa Chiou, Guoping Fan and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative biomaterials, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jun LiuState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.ORCID https://orcid.org/0000-0002-9025-8341
Qian LiuState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.ORCID https://orcid.org/0000-0001-9188-3424
Minmei GuoState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Chengyu JiangState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Jianyang ChenState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Ting WangState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.ORCID https://orcid.org/0000-0002-0961-8224
Tzu-Cheng SungState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Shih-Jie ChouDepartment of Medical Research, Taipei Veterans General Hospital, Taipei 112201, Taiwan, China.
Shih-Hwa ChiouDepartment of Medical Research, Taipei Veterans General Hospital, Taipei 112201, Taiwan, China.ORCID https://orcid.org/0000-0002-2481-5357
Guoping FanDepartment of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
Akon HiguchiState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A variety of novel peptide-grafted hydrogels, of which peptides were derived from vitronectin (PQVTRGDVFTMP) or the laminin β4 chain (PMQKMRGDVFSP), were prepared in this study. The peptide-grafted hydrogels promoted the adhesion, proliferation and colony formation of hiPSCs and maintained their pluripotency up to passage 5 under xeno-free conditions. We successfully generated RPE cells from hiPSCs using one of the most suitable xeno-free peptide-grafted hydrogels, KVN2CK (KGCGGKGG-PQVTRGDVFTMP), which was derived from vitronectin, and confirmed the effect of these hiPSC-derived RPE cells in a rat retinal degeneration model (Royal College of Surgeons (RCS) rats) via subretinal transplantation, when we investigated functional improvements in vision in RCS rats after the transplantation of hiPSC-derived RPE cells. Our novel peptide-grafted hydrogels provided a safe and robust platform for generating single-layer hiPSC-derived RPE cells under xeno-free conditions, which indicates the potential of these hydrogels for stem cell therapy for retinal degenerative diseases in the future.

Indexed as

human pluripotent stem cellhydrogelpeptideretinal pigment epitheliumsubretinal transplantation

Identifiers

PMID40416648
PMCPMC12098265

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.