Evidence map›Paper›PMID 40415954›Full record

ArticleFrontiers in cellular and infection microbiology2025

Human beta defensin-2 protects the epithelial barrier during methicillin-resistant

Tengfei Tian, Szuyao Hsu, Qin Sun, Yang Shi, Xianyang Hu, Yang Wu, Keqing Zhao, Chunquan Zheng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Tengfei Tian *Department of Otolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China.
Szuyao Hsu *Department of Otolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China.
Qin SunDepartment of Otolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China.
Yang ShiDepartment of Otolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China.
Xianyang HuLaboratory for Reproductive Immunology, Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital of Obstetrics and Gynecology, Shanghai Medical College of Fudan University, Shanghai, China.
Yang WuKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College of Fudan University, Shanghai, China.
Keqing ZhaoDepartment of Otolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China.
Chunquan ZhengDepartment of Otolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: We investigated the effect of human beta defensin-2 (hBD-2) on nasal epithelial barrier function with methicillin-resistant Methods: The expression of hBD-2 was measured in nasal polyps (NPs) from CRSwNP. MRSA was treated with different concentrations of hBD-2 to assess the invasive ability. Primary human nasal epithelial cells (HNECs) cultured at the air-liquid interface (ALI) were pre-incubated with or without hBD-2 prior to MRSA infection. The cell viability, the epithelial cell integrity, and the tight junction (TJ) expression were evaluated. Results: The expression of hBD-2 in the CRSwNP group was higher than that in the control group. In addition, the hBD-2 protein was negatively correlated with the Lund-Mackay CT score and was positively correlated with the neutrophil levels in CRSwNP. The presence of hBD-2 significantly reduced the invasive ability of MRSA in HNECs. MRSA decreased the epithelial cell integrity by diminishing the protein expression of occludin and zonula occludens-1 (ZO-1). Furthermore, hBD-2 prevented the MRSA-induced barrier disruption by increasing the mucosal permeability and the expression of occludin and ZO-1. Conclusion: The results suggest that hBD-2 may partially attenuate the epithelial barrier disruption induced by MRSA, indicating the protective effect of hBD-2 on

Indexed as

beta-DefensinsMethicillin-Resistant Staphylococcus aureusNasal PolypsRhinitisSinusitisStaphylococcal InfectionsAdultCells, CulturedCell SurvivalChronic DiseaseEpithelial CellsFemaleHumansMaleMiddle AgedNasal Mucosabeta-DefensinsDEFB4A protein, humanOccludinZonula Occludens-1 Proteinchronic rhinosinusitis with nasal polypsepithelial barrierhuman beta defensin-2methicillin-resistant Staphylococcus aureustight junctions

Identifiers

PMID40415954
PMCPMC12098561

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.