Evidence map›Paper›PMID 40415435›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2025

[RGL1 overexpression promotes metastasis of colorectal cancer by upregulating motile focal adhesion assembly

Nuozhou Weng, Bin Tan, Wentao Zeng, Jiayu Gu, Lianji Weng, Kehong Zheng

Abstract readEnglish Abstract
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Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Nuozhou WengGeneral Surgery Department, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Bin TanGeneral Surgery Department, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Wentao ZengGeneral Surgery Department, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Jiayu GuGeneral Surgery Department, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Lianji WengGeneral Surgery Department, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Kehong ZhengGeneral Surgery Department, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesTo investigate the regulatory role of Ral guanine nucleotide dissociation stimulator-like 1 (RGL1) in metastasis of colorectal cancer (CRC).

methodsWe analyzed the differential expression of RGL1 between metastatic and non-metastatic CRC in GEO database, and examined its expression in 25 patients with metastatic CRC and 25 patients with non-metastatic CRC treated in Zhujiang Hospital between January, 2020 and December, 2022 using quantitative PCR (qPCR) and immunohistochemistry. HCT116 cell lines with stable RGL1 overexpression and SW480 cells with RGL1 knockdown were established using lentiviral vecors, and the changes in invasion and migration abilities of the cells were assessed using Transwell invasion and migration assays. The transduced cells were injected into the serosa of the cecum of nude mice, and tumor growth and liver metastasis were observed 8 weeks later. Fibronectin adhesion assays and immunofluorescence experiments were employed to assess the relationship between RGL1 and focal adhesion formation, and co-immuno-precipitation assays were performed to explore the interaction between RGL1 and GTPase activation.

resultsCompared with non-metastatic CRC, metastatic CRC showed significantly upregulated expression of RGL1. HCT116 cells overexpressing RGL1 exhibited obviously enhanced migration and invasion

conclusionsRGL1 is highly expressed in metastatic CRC and promotes distant metastasis of CRC by activating the CDC42/RAC1 complex to facilitate the formation of motile focal adhesions. These findings suggest that RGL1 can potentially serve as a therapeutic target for CRC metastasis.

Indexed as

Adaptor Proteins, Signal Transducingcdc42 GTP-Binding ProteinColorectal NeoplasmsFocal Adhesionsrac1 GTP-Binding ProteinAnimalsCell Line, TumorCell MovementFemaleHCT116 CellsHumansMiceMice, NudeNeoplasm InvasivenessNeoplasm MetastasisRho Guanine Nucleotide Exchange FactorsAdaptor Proteins, Signal TransducingARHGEF7 protein, humancdc42 GTP-Binding ProteinCDC42 protein, humanrac1 GTP-Binding ProteinRAC1 protein, humanRho Guanine Nucleotide Exchange FactorsCDC42metastatic colorectal cancermotile focal adhesionRAC1Ral guanine nucleotide dissociation stimulator-like 1

Identifiers

PMID40415435
PMCPMC12104737

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