Evidence map›Paper›PMID 40415336›Full record

ArticleBiochemistry2025

A Geranylated Natural Product Simamycin Disrupts the Allosteric Catalysis of tRNA-2-selenouridine Synthase SelU.

Stephen J Dansereau, Alexander Shekhtman, Francesco Epifano, Salvatore Genovese, Serena Fiorito, Thomas J Begley, Jia Sheng

Abstract read
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In one paragraph

Article in Biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

7 authors.

Stephen J DansereauDepartment of Chemistry, University at Albany, State University of New York, 1400 Washington Avenue, Albany, New York 12222, United States.
Alexander ShekhtmanDepartment of Chemistry, University at Albany, State University of New York, 1400 Washington Avenue, Albany, New York 12222, United States.ORCID 0000-0003-2649-2675
Francesco EpifanoDepartment of Pharmacy, University "Gabriele D'Annunzio" of Chieti-Pescara, Chieti Scalo 66100, Italy.
Salvatore GenoveseDepartment of Pharmacy, University "Gabriele D'Annunzio" of Chieti-Pescara, Chieti Scalo 66100, Italy.
Serena FioritoDepartment of Pharmacy, University "Gabriele D'Annunzio" of Chieti-Pescara, Chieti Scalo 66100, Italy.
Thomas J BegleyDepartment of Chemistry, University at Albany, State University of New York, 1400 Washington Avenue, Albany, New York 12222, United States.
Jia ShengDepartment of Chemistry, University at Albany, State University of New York, 1400 Washington Avenue, Albany, New York 12222, United States.ORCID 0000-0001-6198-390X

Funding

Chemical Modifications to Wobble Uridines in tRNA Regulate Responses to StressR01GM143749 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI BEGLEY, THOMAS J, SHENG, JIA · 2022 to 2025
$1.4M
NIGMS NIH HHS R01 GM143749
6 · The paper itself

Abstract

tRNA-2-selenouridine synthase (SelU) is a tRNA-modifying enzyme that is instrumental to bacterial translation by exploiting certain chalcogens. Specifically, this enzyme catalyzes the geranylation of 2-thiouridine at the wobble position of three bacterial tRNAs to enhance the recognition of codons ending in guanosine over adenosine using geranyl pyrophosphate as the cofactor. In addition, SelU is also the working enzyme for a selenation process at the same tRNA position in the presence of selenophosphate. How this enzyme conducts two mechanistically different reactions is a fundamentally interesting question. In order to gain more details about the substrate recognition of SelU, in this work, we identified a small natural compound simamycin (5'-

Indexed as

Amino Acyl-tRNA SynthetasesBacterial ProteinsUridineAllosteric RegulationCatalysisCatalytic DomainKineticsModels, MolecularRNA, TransferAmino Acyl-tRNA SynthetasesBacterial ProteinsRNA, TransferUridine

Identifiers

PMID40415336
PMCPMC12177912

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.