ArticleProtein and peptide letters2025
Molecular Cloning and Expression of Cryptocyanin Gene Isolated from an Indian Variety of
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Abstract
backgroundMolting and reproduction play vital roles in the life cycle of brachyuran crabs, and these two processes are closely interconnected. A key player in the molting cycle is cryptocyanin, which is similar to hemocyanin in sequence, size, and structure. Hemocyanin is a copper-containing oxygen-binding protein, while cryptocyanin is a copper-free protein that lacks oxygen-binding capacity.
objectiveThe goal of the study was to carry out the isolation, cloning, and expression of the partial cryptocyanin gene from the Indian variety of
methodsThe partial cryptocyanin gene was isolated from the hemocytes of the
resultsWe successfully amplified, cloned, and expressed a 519 bp partial cDNA encoding cryptocyanin from the Indian variety of DISCUSSION: In this study, we conducted a comprehensive analysis of cryptocyanin expression in male and female crabs during the intermolt stage. Our findings revealed Cq values of 28.97 for males and 33.68 for females, highlighting a significantly lower abundance of cryptocyanin protein in female crabs.
conclusionOur study showed that crustacean cDNA can be effectively expressed in bacterial vectors, and clones were stable for up to 6 months at -80oC. Real-time data showed a significant difference in cryptocyanin levels between male and female crabs. This finding highlights the need for further research with a larger sample size for better understanding.
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