Evidence map›Paper›PMID 40414999›Full record

ArticleScientific reports2025

Integrating wastewater surveillance for future pandemic preparedness by tracking SARS-CoV-2 RNA in municipal wastewater in Southwest Iran.

Rozhan Feizi, Neematollah Jaafarzadeh, Masoud Panahi Fard, Niloofar Neisi, Ali Zafarzadeh, Mohammad Sabaghan, Ali Veisi

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Rozhan FeiziBehbahan Faculty of Medical Sciences, Behbahan, Iran.
Neematollah JaafarzadehEnvironmental Technologies Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. n.Jaafarzade@yahoo.com.
Masoud Panahi FardStudent Research Committee, Department of Environmental Health Engineering, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. masoudpanahifard@gmail.com.
Niloofar NeisiDepartment of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Ali ZafarzadehEnvironmental Health Research Center, Faculty of Health, Golestan University of Medical Sciences, Gorgan, Iran.
Mohammad SabaghanBehbahan Faculty of Medical Sciences, Behbahan, Iran.
Ali VeisiBehbahan Faculty of Medical Sciences, Behbahan, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Wastewater can be a possible source of virus transmission. Therefore, the present research evaluates the presence of SARS-CoV-2 RNA in untreated municipal wastewater in Behbahan County, Iran. 24 wastewater samples were collected from January to February 2021. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was applied for the detection of RNA of the virus of interest. Based on the results, SARS-CoV-2 RNA was detected with concentrations ranging from 0 to 2100 gene copies/L in studied samples. A strong positive correlation (r = 0.76, p < 0.01) was observed between viral RNA concentrations in wastewater and the number of confirmed COVID-19 cases in the region. Based on the findings, wastewater-based epidemiology (WBE) can be used as a cost-effective and non-invasive tool for monitoring community-level viral transmission. This research highlights the importance of using WBE in public health strategies, particularly in resource-limited settings, to complement clinical surveillance and provide early warnings of outbreaks. Further research is required to investigate seasonal variations in virus shedding, optimize detection methods to improve sensitivity, and further investigate the relationship between wastewater viral loads and disease transmission dynamics to enhance pandemic preparedness and response.

Indexed as

COVID-19RNA, ViralSARS-CoV-2WastewaterHumansIranPandemic PreparednessPandemicsWastewater-Based Epidemiological MonitoringRNA, ViralWastewaterIranPublic healthRNA detectionRT-qPCRSARS-CoV-2Wastewater surveillance

Identifiers

PMID40414999
PMCPMC12104335

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.