Evidence map›Paper›PMID 40414903›Full record

ArticleScientific reports2025

Valorization of wood waste for enhanced polyhydroxybutyrate production by Klebsiella sp. MK3.

Mukesh Kumar, Rohan Samir Kumar Sachan, Arun Karnwal, Dharmesh Sur, Rahul Thakur, Abhinav Kumar, Sathiyamoorthy Manickkam, Abinet Gosaye Ayanie, Hamad Almujibah

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mukesh KumarDepartment of Microbiology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, 144411, Punjab, India. mk.narwat111@gmail.com.
Rohan Samir Kumar SachanDepartment of Medical Laboratory Sciences, GNA University, Phagwara, 144401, Punjab, India.
Arun KarnwalDepartment of Microbiology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, 144411, Punjab, India.
Dharmesh SurDepartment of Chemical Engineering, Faculty of Engineering & Technology, Marwadi University Research Center, Marwadi University, Rajkot, 360003, Gujarat, India.
Rahul ThakurDepartment of Chemistry, Chandigarh Engineering College, Chandigarh Group of Colleges-Jhanjeri, Mohali, 140307, Punjab, India.
Abhinav KumarDepartment of Nuclear and Renewable Energy, Ural Federal University Named after the First President of Russia Boris Yeltsin, Ekaterinburg, 620002, Russia.
Sathiyamoorthy ManickkamFaculty-Chemical Engineering, Higher Colleges of Technology, Ruwais Women's College, Abu Dhabi, United Arab Emirates.
Abinet Gosaye AyanieDepartment of Mechanical Engineering, Adama Science and Technology University, Adama, 2552, Ethiopia. abinet.gosaye@astu.edu.et.
Hamad AlmujibahDepartment of Civil Engineering, College of Engineering, Taif University, P.O. Box 11099, Taif City, 21974, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The valorization of wood waste as a sustainable bacterial feedstock for the production of Polyhydroxybutyrate (PHB) is explored in this study, aiming to provide an environmentally friendly alternative to conventional plastics. Wood waste, treated with 4% sulfuric acid, served as the carbon source for isolating bacteria from Jalandhar waste streams, with the strain Klebsiella sp. MK3 identified as the most effective in PHB production after 16s rRNA sequencing. Analytical methods including the Molisch test, DNS, and sugar utilization tests confirmed sugar presence and consumption by the bacterial isolate. Media optimization using Design Expert 12.0 utilized a quadratic model, achieving a robust fit with an R² value of 98.6%. Optimization via Plackett-Burman design and response surface methodology enhanced PHB yield to 4.37 mg/mL, a significant increase over previous benchmarks. This yield was achieved under optimal conditions of 1.7% carbon concentration, 0.105% nitrogen concentration, and a constant temperature of 37 °C. Qualitative analysis of PHB by UV-Vis spectroscopy, FTIR, and NMR confirmed its purity and composition. The study highlights the potential of wood waste and wastewater as substrates for cost-effective PHB production, with significant applications in packaging, agriculture, medicine, and more, thus promoting reduced reliance on non-renewable resources and advancing sustainability goals.

Indexed as

HydroxybutyratesKlebsiellaPolyestersWoodCarbonPolyhydroxybutyratesRNA, Ribosomal, 16SCarbonHydroxybutyratesPolyesterspolyhydroxybutyratePolyhydroxybutyratesRNA, Ribosomal, 16SKlebsiella sp. MK3Plackett-Burman design (PBD)Polyhydroxybutyrate (PHB)Response surface methodology (RSM)Statistical experimental design.Wood waste valorization

Identifiers

PMID40414903
PMCPMC12104425

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.